BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6506

Title: Conkunitzin-S1 is the first member of a new Kunitz-type neurotoxin family - Structural and functional characterization   PubMed: 15833744

Deposition date: 2005-02-17 Original release date: 2005-08-22

Authors: Bayrhuber, M.; Vijayan, V.; Ferber, M.; Graf, R.; Korukottu, J.; Olivera, B.; Terlau, H.; Zweckstetter, M.; Becker, S.

Citation: Bayrhuber, M.; Vijayan, V.; Ferber, M.; Graf, R.; Korukottu, J.; Imperial, J.; Garrett, J.; Olivera, B.; Terlau, H.; Zweckstetter, M.; Becker, S.. "Conkunitzin-S1 Is the First Member of a New Kunitz-type Neurotoxin Family: STRUCTURAL AND FUNCTIONAL CHARACTERIZATION."  J. Biol. Chem. 280, 23766-23770 (2005).

Assembly members:
Conkunitzin-S1, polymer, 60 residues, Formula weight is not available

Natural source:   Common Name: striated cone   Taxonomy ID: 6493   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus striatus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Conkunitzin-S1: KDRPSLCDLPADSGSGTKAE KRIYYNSARKQCLRFDYTGQ GGNENNFRRTYDCQRTCLYT

Data sets:
Data typeCount
13C chemical shifts209
15N chemical shifts55
1H chemical shifts302

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Conkunitzin-S11

Entities:

Entity 1, Conkunitzin-S1 60 residues - Formula weight is not available

1   LYSASPARGPROSERLEUCYSASPLEUPRO
2   ALAASPSERGLYSERGLYTHRLYSALAGLU
3   LYSARGILETYRTYRASNSERALAARGLYS
4   GLNCYSLEUARGPHEASPTYRTHRGLYGLN
5   GLYGLYASNGLUASNASNPHEARGARGTHR
6   TYRASPCYSGLNARGTHRCYSLEUTYRTHR

Samples:

sample_1: Conkunitzin-S1, [U-15N; U-13C], 1 mM; sodium acetate buffer 100 mM; H2O 90%; D2O 10%

sample_2: Conkunitzin-S1, [U-15N], 0.5 mM; sodium acetate buffer 100 mM; H2O 90%; D2O 10%

sample_3: Conkunitzin-S1, [U-15N; U-13C], 0.5 mM; sodium acetate buffer 100 mM

conditions_1: pH: 5.2; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availableconditions_1

Software:

xwinnmr v3.5 - collection

NMRPipe v97.027.12.56 - processing

NMRDraw v2.3 Rev 2004.245.17.31 - processing

SPARKY v3.110 - data analysis

ARIA v2alpha - structure solution

MARS v1.0 - data analysis

CYANA v2.0.32 - refinement

NMR spectrometers:

  • Bruker AVANCE 900 MHz

Related Database Links:

PDB
GB AHG24896
SP P0C1X2
AlphaFold P0C1X2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts