BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6692

Title: 1H, 13C, and 15N Chemical Shift Assignments for AppA 5-125   PubMed: 16323221

Deposition date: 2005-06-16 Original release date: 2007-02-05

Authors: Grinstead, Jeffrey; Hsu, Shang-Te; Laan, Wouter; Bonvin, Alexandre; Hellingwerf, Klaas; Boelens, Rolf; Kaptein, Rob

Citation: Grinstead, Jeffrey; Hsu, Shang-Te; Laan, Wouter; Bonvin, Alexandre; Hellingwerf, Klaas; Boelens, Rolf; Kaptein, Robert. "The Solution Structure of the AppA BLUF Domain: Insight into the Mechanism of Light-Induced Signaling"  Chem. Biochem. 7, 187-193 (2006).

Assembly members:
AppA, polymer, 121 residues, Formula weight is not available
FAD, non-polymer, 785.550 Da.

Natural source:   Common Name: Rhodobacter sphaeroides   Taxonomy ID: 1063   Superkingdom: Eubacteria   Kingdom: Not applicable   Genus/species: Rhodobacter sphaeroides

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
AppA: LEADVTMTGSDLVSCCYRSL AAPDLTLRDLLDIVETSQAH NARAQLTGALFYSQGVFFQW LEGHPAAVAEVMSHIQRDRR HSNVEILAEESIAKRRFAGW HMQLSCSEADMRSLGLAESR Q

Data sets:
Data typeCount
13C chemical shifts349
15N chemical shifts128
1H chemical shifts798

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AppA1
2FAD2

Entities:

Entity 1, AppA 121 residues - Formula weight is not available

1   LEUGLUALAASPVALTHRMETTHRGLYSER
2   ASPLEUVALSERCYSCYSTYRARGSERLEU
3   ALAALAPROASPLEUTHRLEUARGASPLEU
4   LEUASPILEVALGLUTHRSERGLNALAHIS
5   ASNALAARGALAGLNLEUTHRGLYALALEU
6   PHETYRSERGLNGLYVALPHEPHEGLNTRP
7   LEUGLUGLYHISPROALAALAVALALAGLU
8   VALMETSERHISILEGLNARGASPARGARG
9   HISSERASNVALGLUILELEUALAGLUGLU
10   SERILEALALYSARGARGPHEALAGLYTRP
11   HISMETGLNLEUSERCYSSERGLUALAASP
12   METARGSERLEUGLYLEUALAGLUSERARG
13   GLN

Entity 2, FAD - C27 H33 N9 O15 P2 - 785.550 Da.

1   FAD

Samples:

sample_1: AppA, [U-13C; U-15N], 1.7 mM; FAD 1.7 mM

sample_2: AppA, [U-15N], 1.7 mM; FAD mM

conditions_1: pH: 7.4; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
1H15N HSQCnot availablenot availableconditions_1
triple resonancenot availablenot availableconditions_1
hCCH-COSYnot availablenot availableconditions_1
HcCH-TOCSYnot availablenot availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker AVANCE 600 MHz
  • Bruker AVANCE 700 MHz

Related Database Links:

PDB
GB AAA81591 ABA77707 ABN75335 ACM02822 EGJ23059
REF WP_002722303 WP_009564586 WP_011336839 WP_011840246 WP_015921786

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts