BMRB Entry 6811
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6811
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NMR-STAR v3 text file.
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Title: Solution structure of apoCadA PubMed: 16388822
Deposition date: 2005-09-07 Original release date: 2007-01-26
Authors: Banci, L.; Bertini, I.; Ciofi-Baffoni, S.; Su, X.-C.; Miras, R.; Bal, N.; Mintz, E.; Catty, P.; Shokes, J.; Scott, R.
Citation: Banci, L.; Bertini, I.; Ciofi-Baffoni, S.; Su, X.-C.; Miras, R.; Bal, N.; Mintz, E.; Catty, P.; Shokes, J.; Scott, R.. "Structural Basis for Metal Binding Specificity: the N-terminal Cadmium Binding Domain of the P1-type ATPase CadA." J. Mol. Biol. 356, 638-650 (2005).
Assembly members:
P-type ATPase, polymer, 71 residues, Formula weight is not available
Natural source: Common Name: Listeria monocytogenes Taxonomy ID: 1639 Superkingdom: Eubacteria Kingdom: not applicable Genus/species: Listeria monocytogenes
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
P-type ATPase: MAEKTVYRVDGLSCTNCAAK
FERNVKEIEGVTEAIVNFGA
SKITVTGEASIQQVEQAGAF
EHLKIIPEKEA
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 75 |
| 1H chemical shifts | 458 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Cadmium-transporting ATPase | 1 |
Entities:
Entity 1, Cadmium-transporting ATPase 71 residues - Formula weight is not available
| 1 | MET | ALA | GLU | LYS | THR | VAL | TYR | ARG | VAL | ASP | ||||
| 2 | GLY | LEU | SER | CYS | THR | ASN | CYS | ALA | ALA | LYS | ||||
| 3 | PHE | GLU | ARG | ASN | VAL | LYS | GLU | ILE | GLU | GLY | ||||
| 4 | VAL | THR | GLU | ALA | ILE | VAL | ASN | PHE | GLY | ALA | ||||
| 5 | SER | LYS | ILE | THR | VAL | THR | GLY | GLU | ALA | SER | ||||
| 6 | ILE | GLN | GLN | VAL | GLU | GLN | ALA | GLY | ALA | PHE | ||||
| 7 | GLU | HIS | LEU | LYS | ILE | ILE | PRO | GLU | LYS | GLU | ||||
| 8 | ALA |
Samples:
sample_1: P-type ATPase, [U-15N], 2 mM; sodium phosphate buffer 350 mM; TCEP 5 mM; H2O 90%; D2O 10%
sample_cond_1: ionic strength: 350 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | not available |
| 2D TOCSY | not available | not available | not available |
| HNHA | not available | not available | not available |
| HNHB | not available | not available | not available |
| 3D 15N-separated NOESY | not available | not available | not available |
Software:
xwinnmr - collection, processing
NEASY - data analysis
DYANA - structure solution
AMBER v5.0 - refinement
NMR spectrometers:
- Bruker AVANCE 500 MHz
Related Database Links:
| PDB | |
| EMBL | CBV37084 CBV37138 CBV37201 CCO11808 CDM15195 |
| GB | AAA25275 ADB69774 ADI61866 AGR27763 AGT06861 |
| REF | WP_003728466 WP_015076936 WP_031644883 YP_003415136 YP_003728018 |
| SP | Q60048 |
| AlphaFold | Q60048 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts