BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6872

Title: Solution structure of kalata B8   PubMed: 16207177

Deposition date: 2005-10-20 Original release date: 2008-09-24

Authors: Daly, N.; Clark, R.; Plan, M.; Craik, D.

Citation: Daly, N.; Clark, R.; Plan, M.; Craik, D.. "Kalata B8, a novel antiviral circular protein, exhibits conformational flexibility in the cystine knot motif"  Biochem. J. 393, 619-626 (2006).

Assembly members:
kalata B8, polymer, 31 residues, Formula weight is not available

Natural source:   Common Name: Oldenlandia affinis   Taxonomy ID: 60225   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Oldenlandia affinis

Experimental source:   Production method: recombinate technology

Entity Sequences (FASTA):
kalata B8: CGETCLLGTCYTTGCTCNKY RVCTKDGSVLN

Data sets:
Data typeCount
1H chemical shifts183

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1kalata B81

Entities:

Entity 1, kalata B8 31 residues - Formula weight is not available

1   CYSGLYGLUTHRCYSLEULEUGLYTHRCYS
2   TYRTHRTHRGLYCYSTHRCYSASNLYSTYR
3   ARGVALCYSTHRLYSASPGLYSERVALLEU
4   ASN

Samples:

sample_1: kalata B8 1 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 3.5; pressure: 1 atm; temperature: 290 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available
E-COSYnot availablenot availablenot available

Software:

xwinnmr v3.5 - collection

XEASY v1.3.7 - data analysis

DYANA v1.5 - refinement

CNS v1.1 - structure solution

NMR spectrometers:

  • Bruker AVANCE 500 MHz

Related Database Links:

PDB
SWISS-PROT P85175