BMRB Entry 7082
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7082
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Title: 1H, 13C, and 15N assignments for the Psuedomonas putida protein PutA45 PubMed: 18767154
Deposition date: 2006-04-21 Original release date: 2009-05-27
Authors: Halouska, Steven; Zhou, Yuzhen; Becker, Donald; Powers, Robert
Citation: Halouska, Steven; Zhou, Yuzhen; Becker, Donald; Powers, Robert. "Solution Structure of the Pseudomonas putida protein PpPutA45 and its DNA complex" Proteins 75, 12-27 (2009).
Assembly members:
PutA45, polymer, 51 residues, 6007 Da.
Natural source: Common Name: Psuedomonas putida Taxonomy ID: 303 Superkingdom: Eubacteria Kingdom: not available Genus/species: Psuedomonas putida
Experimental source: Production method: Purified from the natural source
Entity Sequences (FASTA):
PutA45: MATTTLGVKLDDPTRERLKA
AAQSIDRTPHWLIKQAIFNY
LEKLEHHHHHH
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 200 |
15N chemical shifts | 42 |
1H chemical shifts | 316 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | PutA45 unit1 | 1 |
2 | PutA45 unit2 | 1 |
Entities:
Entity 1, PutA45 unit1 51 residues - 6007 Da.
1 | MET | ALA | THR | THR | THR | LEU | GLY | VAL | LYS | LEU | ||||
2 | ASP | ASP | PRO | THR | ARG | GLU | ARG | LEU | LYS | ALA | ||||
3 | ALA | ALA | GLN | SER | ILE | ASP | ARG | THR | PRO | HIS | ||||
4 | TRP | LEU | ILE | LYS | GLN | ALA | ILE | PHE | ASN | TYR | ||||
5 | LEU | GLU | LYS | LEU | GLU | HIS | HIS | HIS | HIS | HIS | ||||
6 | HIS |
Samples:
sample_1: PutA45, [U-99% 13C, U-99% 15 N], 2.2 mM; phospate buffer 50 mM; NaCl 200 mM; D2O 10%; H2O 90%
conditions_1: ionic strength: 200 mM; pH: 6.2; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
HSQC | sample_1 | not available | conditions_1 |
HNCO | sample_1 | not available | conditions_1 |
HNCA | sample_1 | not available | conditions_1 |
CBCANH | sample_1 | not available | conditions_1 |
CBCACONH | sample_1 | not available | conditions_1 |
HCCCONH | sample_1 | not available | conditions_1 |
CCCONH | sample_1 | not available | conditions_1 |
C13-NOESY | sample_1 | not available | conditions_1 |
N15-NOESY | sample_1 | not available | conditions_1 |
HNHA | sample_1 | not available | conditions_1 |
Software:
NMRPipe v2.2 -
PIPP v4.3.5 -
AutoAssign v1.11.1 -
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
PDB | |
DBJ | BAN56864 BAO59856 |
EMBL | CAK17638 CAY46727 CDF97094 |
GB | AAF25000 AAF73193 AAN70514 AAO58445 AAY95904 |
REF | NP_747050 NP_794750 WP_002551755 WP_003171271 WP_003186541 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts