BMRB Entry 7234

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7234

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Title:
Backbone 1H, 15N, and 13C assignments for beta phosphoglucomutase in a ternary complex with glucose-6-phosphate and MgF3-
Deposition date:
2006-07-13
Original release date:
2006-09-08
Authors:
Baxter, Nicola; Waltho, Jonathan
Citation:

Citation: Baxter, Nicola; Olguin, Luis; Golicnik, Marko; Feng, Guoqiang; Hounslow, Andrea; Bermel, Wolfgang; Blackburn, G.; Hollfelder, Florian; Waltho, Jonathan; Williams, Nicholas. "A trojan horse transition state analogue generated by MgF3- formation in an enzyme active site"  Proc. Natl. Acad. Sci. U.S.A. 103, 14732-14737 (2006).
PubMed: 16990434

Assembly members:

Assembly members:
beta phosphoglucomutase, polymer, 221 residues, 25000 Da.
BG6, non-polymer, 260.136 Da.
MGF, non-polymer, 81.300 Da.

Natural source:

Natural source:   Common Name: Lactococcus lactis   Taxonomy ID: 1360   Superkingdom: Eubacteria   Kingdom: Metazoa   Genus/species: Lactococcus lactis

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
beta phosphoglucomutase: MFKAVLFDLDGVITDTAEYH FRAWKALAEEIGINGVDRQF NEQLKGVSREDSLQKILDLA DKKVSAEEFKELAKRKNDNY VKMIQDVSPADVYPGILQLL KDLRSNKIKIALASASKNGP FLLERMNLTGYFDAIADPAE VAASKPAPDIFIAAAHAVGV APSESIGLEDSQAGIQAIKD SGALPIGVGRPEDLGDDIVI VPDTSHYTLEFLKEVWLQKQ K

Data sets:
Data typeCount
13C chemical shifts646
15N chemical shifts210
1H chemical shifts210

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1beta phosphoglucomutase1
2glucose-6-phosphate2
3magnesium trifluoride ion3

Entities:

Entity 1, beta phosphoglucomutase 221 residues - 25000 Da.

1   METPHELYSALAVALLEUPHEASPLEUASP
2   GLYVALILETHRASPTHRALAGLUTYRHIS
3   PHEARGALATRPLYSALALEUALAGLUGLU
4   ILEGLYILEASNGLYVALASPARGGLNPHE
5   ASNGLUGLNLEULYSGLYVALSERARGGLU
6   ASPSERLEUGLNLYSILELEUASPLEUALA
7   ASPLYSLYSVALSERALAGLUGLUPHELYS
8   GLULEUALALYSARGLYSASNASPASNTYR
9   VALLYSMETILEGLNASPVALSERPROALA
10   ASPVALTYRPROGLYILELEUGLNLEULEU
11   LYSASPLEUARGSERASNLYSILELYSILE
12   ALALEUALASERALASERLYSASNGLYPRO
13   PHELEULEUGLUARGMETASNLEUTHRGLY
14   TYRPHEASPALAILEALAASPPROALAGLU
15   VALALAALASERLYSPROALAPROASPILE
16   PHEILEALAALAALAHISALAVALGLYVAL
17   ALAPROSERGLUSERILEGLYLEUGLUASP
18   SERGLNALAGLYILEGLNALAILELYSASP
19   SERGLYALALEUPROILEGLYVALGLYARG
20   PROGLUASPLEUGLYASPASPILEVALILE
21   VALPROASPTHRSERHISTYRTHRLEUGLU
22   PHELEULYSGLUVALTRPLEUGLNLYSGLN
23   LYS

Entity 2, glucose-6-phosphate - C6 H13 O9 P - 260.136 Da.

1   BG6

Entity 3, magnesium trifluoride ion - F3 Mg - 81.300 Da.

1   MGF

Related Database Links:

BMRB 15467 16409 17851 17852 7235
PDB 1LVH 1O03 1O08 1Z4N 1Z4O 1ZOL 2WF5 2WF6 2WF7 2WF8 2WF9 2WFA 2WHE 3FM9 3ZI4 4C4R 4C4S 4C4T
DBJ BAL50396
EMBL CAA94734 CDG03643 CDI46177
GB AAK04527 ADA64250 ADZ63078 AEE43918 AGY43735
REF NP_266585 WP_003131530 WP_010905331 WP_012897250 WP_021469610
SP P71447
AlphaFold P71447

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks