BMRB Entry 10005
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10005
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Title: 1H, 13C, and 15N Chemical Shift Assignments for ChitinaseC chitin binding domain PubMed: 16567413
Deposition date: 2005-10-12 Original release date: 2007-06-13
Authors: Akagi, Ken-ichi; Ikegami, Takahisa
Citation: Akagi, Ken-ichi; Watanabe, Jun; Hara, Masashi; Kezuka, Yuichiro; Chikaishi, Eriko; Yamaguchi, Tohru; Akutsu, Hideo; Nonaka, Takamasa; Watanabe, Takeshi; Takahisa, Ikegami. "Identification of the Substrate Interaction Region of the Chitin-Binding Domain of Streptomyces griseus Chitinase C" J. Biochem. 139, 483-493 (2006).
Assembly members:
ChitinaseC chitin binding domain, polymer, 54 residues, 5741 Da.
Natural source: Common Name: Streptomyces griseinus Taxonomy ID: 66893 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptomyces griseinus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
ChitinaseC chitin binding domain: MATCATAWSSSSVYTNGGTV
SYNGRNYTAKWWTQNERPGT
SDVWADKGACGTGS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 188 |
| 15N chemical shifts | 61 |
| 1H chemical shifts | 301 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ChBD | 1 |
Entities:
Entity 1, ChBD 54 residues - 5741 Da.
| 1 | MET | ALA | THR | CYS | ALA | THR | ALA | TRP | SER | SER | ||||
| 2 | SER | SER | VAL | TYR | THR | ASN | GLY | GLY | THR | VAL | ||||
| 3 | SER | TYR | ASN | GLY | ARG | ASN | TYR | THR | ALA | LYS | ||||
| 4 | TRP | TRP | THR | GLN | ASN | GLU | ARG | PRO | GLY | THR | ||||
| 5 | SER | ASP | VAL | TRP | ALA | ASP | LYS | GLY | ALA | CYS | ||||
| 6 | GLY | THR | GLY | SER |
Samples:
sample_1: ChitinaseC chitin binding domain, [U-13C; U-15N], 1.0 mM
condition_1: pH: 6.0; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | sample_1 | not available | condition_1 |
| 1H-13C HSQC | sample_1 | not available | condition_1 |
| 1H-1H TOCSY | sample_1 | not available | condition_1 |
| 1H-1H NOESY | sample_1 | not available | condition_1 |
| NOESY-15N-HSQC | sample_1 | not available | condition_1 |
| TOCSY-15N-HSQC | sample_1 | not available | condition_1 |
| HNCO | sample_1 | not available | condition_1 |
| HN(CA)CO | sample_1 | not available | condition_1 |
| CBCA(CO)NH | sample_1 | not available | condition_1 |
| CBCANH | sample_1 | not available | condition_1 |
| HBHA(CBCACO)NH | sample_1 | not available | condition_1 |
| HCCH-TOCSY | sample_1 | not available | condition_1 |
| C(CO)NH | sample_1 | not available | condition_1 |
| H(CCO)NH | sample_1 | not available | condition_1 |
| (HB)CB(CGCD)HD | sample_1 | not available | condition_1 |
| (HB)CB(CGCDCE)HE | sample_1 | not available | condition_1 |
Software:
No software information available
NMR spectrometers:
- Bruker DRX500 500 MHz
- Bruker DRX600 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA23739 BAG20229 |
| GB | ADQ43777 EGE43002 KND35043 KPL31411 KQX37120 |
| REF | WP_003967541 WP_026291187 WP_050358467 |
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