BMRB Entry 10030

Name: Solution structure of the forth CH domain from human plastin 3 T-isoform
Published: 2008-08-12

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PDB ID: 1wjo
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10030
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the forth CH domain from human plastin 3 T-isoform

Deposition date:

2006-11-01

Original release date:

2008-08-12

Authors:

Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the forth CH domain from human plastin 3 T-isoform" .

Assembly members:

Assembly members:
CH domain, polymer, 124 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P040223-07

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CH domain: GSSGSSGNDDIIVNWVNRTL SEAGKSTSIQSFKDKTISSS LAVVDLIDAIQPGCINYDLV KSGNLTEDDKHNNAKYAVSM ARRIGARVYALPEDLVEVKP KMVMTVFACLMGRGMKRVSG PSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	520
15N chemical shifts	127
1H chemical shifts	827

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	T-plastin	1

Entities:

Entity 1, T-plastin 124 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ASN

ASP

2

ILE

VAL

ASN

TRP

VAL

ASN

ARG

THR

LEU

3

SER

GLU

ALA

GLY

LYS

SER

THR

SER

ILE

GLN

4

SER

PHE

LYS

ASP

LYS

THR

ILE

SER

5

LEU

ALA

VAL

ASP

LEU

ILE

ASP

ALA

ILE

6

GLN

PRO

GLY

CYS

ILE

ASN

TYR

ASP

LEU

VAL

7

LYS

SER

GLY

ASN

LEU

THR

GLU

ASP

LYS

8

HIS

ASN

ALA

LYS

TYR

ALA

VAL

SER

MET

9

ALA

ARG

ILE

GLY

ALA

ARG

VAL

TYR

ALA

10

LEU

PRO

GLU

ASP

LEU

VAL

GLU

VAL

LYS

PRO

11

LYS

MET

VAL

MET

THR

VAL

PHE

ALA

CYS

LEU

12

MET

GLY

ARG

GLY

MET

LYS

ARG

VAL

SER

GLY

13

PRO

SER

GLY

Samples:

sample_1: CH domain, [U-13C; U-15N], 1.16 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	not available	condition_1
3D 15N-separated NOESY	sample_1	not available	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.901, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1WJO
DBJ	BAD96521 BAF83883 BAG35308 BAG57642 BAG57725
GB	AAB02844 AAH08588 AAH39049 AAH56898 AAI50495
REF	NP_001129497 NP_001165806 NP_001253150 NP_001269266 NP_001269267
SP	P13797
AlphaFold	P13797