BMRB Entry 10062
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR10062
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Backbone 1H and 15N Chemical Shift Assignments for Nedd8 PubMed: 17206147
Deposition date: 2006-12-07 Original release date: 2007-03-23
Authors: Sakata, Eri; Yamaguchi, Yoshiki; Miyauchi, Yasuhiro; Iwai, Kazuhiro; Chiba, Tomoki; Saeki, Yasusi; Matsuda, Noriyuki; Tanaka, Keiji; Kato, Koichi
Citation: Sakata, Eri; Yamaguchi, Yoshiki; Miyauchi, Yasuhiro; Iwai, Kazuhiro; Chiba, Tomoki; Saeki, Yasusi; Matsuda, Noriyuki; Tanaka, Keiji; Kato, Koichi. "Direct interactions between Nedd8 and ubiquitin E2 conjugating enzymes upregulate cullin-based E3 ligase activity" Nat. Struct. Mol. Biol. 14, 167-168 (2007).
Assembly members:
Nedd8, polymer, 96 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Nedd8: MGSSHHHHHHSSGLVPRGSH
MLIKVKTLTGKEIEIDIEPT
DKVERIKERVEEKEGIPPQQ
QRLIYSGKQMNDEKTAADYK
ILGGSVLHLVLALRGG
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 72 |
| 1H chemical shifts | 72 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Nedd8 monomer | 1 |
Entities:
Entity 1, Nedd8 monomer 96 residues - Formula weight is not available
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | MET | LEU | ILE | LYS | VAL | LYS | THR | LEU | THR | GLY | ||||
| 4 | LYS | GLU | ILE | GLU | ILE | ASP | ILE | GLU | PRO | THR | ||||
| 5 | ASP | LYS | VAL | GLU | ARG | ILE | LYS | GLU | ARG | VAL | ||||
| 6 | GLU | GLU | LYS | GLU | GLY | ILE | PRO | PRO | GLN | GLN | ||||
| 7 | GLN | ARG | LEU | ILE | TYR | SER | GLY | LYS | GLN | MET | ||||
| 8 | ASN | ASP | GLU | LYS | THR | ALA | ALA | ASP | TYR | LYS | ||||
| 9 | ILE | LEU | GLY | GLY | SER | VAL | LEU | HIS | LEU | VAL | ||||
| 10 | LEU | ALA | LEU | ARG | GLY | GLY |
Samples:
sample_1: Nedd8, [U-98% 13C; U-98% 15N], 1 mM; MES 50 mM
sample_condition_1: pH: 6.0; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- Bruker DMX500 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAA01719 BAA04889 BAE27394 BAE27660 BAE34759 |
| EMBL | CAF89342 CAF97715 CAG05755 CAG28590 CAJ82981 |
| GB | AAC64189 AAF73911 AAH04625 AAH55645 AAH76245 |
| REF | NP_001002557 NP_001016973 NP_001075681 NP_001154087 NP_001187300 |
| SP | P29595 P61282 Q15843 Q4PLJ0 Q71UE8 |
| TPG | DAA25711 |
| AlphaFold | P29595 P61282 Q15843 Q4PLJ0 Q71UE8 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts