BMRB Entry 10083

Name: PDZ domain of human RIM2B
Published: 2008-08-14

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PDB ID: 1wfg
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10083
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

PDZ domain of human RIM2B

Deposition date:

2007-01-24

Original release date:

2008-08-14

Authors:

Nagashima, T.; Hayashi, F.; Yokoyama, S.

Citation:

Citation: Nagashima, T.; Hayashi, F.; Yokoyama, S.. "PDZ domain of human RIM2B" .

Assembly members:

Assembly members:
PDZ domain, polymer, 131 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P021030-35

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PDZ domain: GSSGSSGHSHSDKHPVTWQP SKDGDRLIGRILLNKRLKDG SVPRDSGAMLGLKVVGGKMT ESGRLCAFITKVKKGSLADT VGHLRPGDEVLEWNGRLLQG ATFEEVYNIILESKPEPQVE LVVSRSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	561
15N chemical shifts	136
1H chemical shifts	899

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Regulating synaptic membrane exocytosis protein 2	1

Entities:

Entity 1, Regulating synaptic membrane exocytosis protein 2 131 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

HIS

SER

HIS

2

SER

ASP

LYS

HIS

PRO

VAL

THR

TRP

GLN

PRO

3

SER

LYS

ASP

GLY

ASP

ARG

LEU

ILE

GLY

ARG

4

ILE

LEU

ASN

LYS

ARG

LEU

LYS

ASP

GLY

5

SER

VAL

PRO

ARG

ASP

SER

GLY

ALA

MET

LEU

6

GLY

LEU

LYS

VAL

GLY

LYS

MET

THR

7

GLU

SER

GLY

ARG

LEU

CYS

ALA

PHE

ILE

THR

8

LYS

VAL

LYS

GLY

SER

LEU

ALA

ASP

THR

9

VAL

GLY

HIS

LEU

ARG

PRO

GLY

ASP

GLU

VAL

10

LEU

GLU

TRP

ASN

GLY

ARG

LEU

GLN

GLY

11

ALA

THR

PHE

GLU

VAL

TYR

ASN

ILE

12

LEU

GLU

SER

LYS

PRO

GLU

PRO

GLN

VAL

GLU

13

LEU

VAL

SER

ARG

SER

GLY

PRO

SER

14

GLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.17 mM; PiNa 20 mM; NaCl 100 mM; DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	not available	condition_1
3D 15N-separated NOESY	sample_1	not available	condition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.8992, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

Varian INOVA 800 MHz

PDB	1WFG
DBJ	BAA34471 BAB18975 BAC98016 BAG10377 BAG54403
EMBL	CAH18227
GB	AAC19157 AAF81644 AAF81645 AAF81646 AAF81647
REF	NP_001093587 NP_001243311 NP_001243312 NP_001269810 NP_055492
SP	Q9EQZ7 Q9JIS1 Q9UQ26
AlphaFold	Q9EQZ7 Q9JIS1 Q9UQ26