BMRB Entry 10124

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PDB ID: 1ujv
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10124
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the second PDZ domain of human membrane associated guanylate kinase inverted-2 (MAGI-2)

Deposition date:

2007-04-02

Original release date:

2008-09-02

Authors:

Nameki, N.; Koshiba, S.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Nameki, N.; Koshiba, S.; Kigawa, T.; Yokoyama, S.. "Solution structure of the second PDZ domain of human membrane associated guanylate kinase inverted-2 (MAGI-2)" .

Assembly members:

Assembly members:
PDZ domain, polymer, 96 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P021030-28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PDZ domain: GSSGSSGQAELMTLTIVKGA QGFGFTIADSPTGQRVKQIL DIQGCPGLCEGDLIVEINQQ NVQNLSHTEVVDILKDCPIG SETSLIIHRGSGPSSG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	366
15N chemical shifts	95
1H chemical shifts	614

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MEMBRANE ASSOCIATED GUANYLATE KINASE INVERTED-2 (MAGI-2)	1

Entities:

Entity 1, MEMBRANE ASSOCIATED GUANYLATE KINASE INVERTED-2 (MAGI-2) 96 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

GLN

ALA

GLU

2

LEU

MET

THR

LEU

THR

ILE

VAL

LYS

GLY

ALA

3

GLN

GLY

PHE

GLY

PHE

THR

ILE

ALA

ASP

SER

4

PRO

THR

GLY

GLN

ARG

VAL

LYS

GLN

ILE

LEU

5

ASP

ILE

GLN

GLY

CYS

PRO

GLY

LEU

CYS

GLU

6

GLY

ASP

LEU

ILE

VAL

GLU

ILE

ASN

GLN

7

ASN

VAL

GLN

ASN

LEU

SER

HIS

THR

GLU

VAL

8

VAL

ASP

ILE

LEU

LYS

ASP

CYS

PRO

ILE

GLY

9

SER

GLU

THR

SER

LEU

ILE

HIS

ARG

GLY

10

SER

GLY

PRO

SER

GLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.3 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.811, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - structure solution

OPALp, Billeter, M., Guntert, P., Koradi, R. - refinement

NMR spectrometers:

Bruker AVANCE 800 MHz