BMRB Entry 10142

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PDB ID: 2e9i
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10142
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the N-terminal extended 20th Filamin domain from human Filamin-B

Deposition date:

2007-12-04

Original release date:

2008-12-10

Authors:

Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the N-terminal extended 20th Filamin domain from human Filamin-B" .

Assembly members:

Assembly members:
filamin domain, polymer, 106 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P060515-02

Entity Sequences (FASTA):

Entity Sequences (FASTA):
filamin domain: GSSGSSGRVKESITRTSRAP SVATVGSICDLNLKIPEINS SDMSAHVTSPSGRVTEAEIV PMGKNSHCVRFVPQEMGVHT VSVKYRGQHVTGSPFQFTVG PLGEGG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	427
15N chemical shifts	96
1H chemical shifts	670

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Filamin-B	1

Entities:

Entity 1, Filamin-B 106 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ARG

VAL

LYS

2

GLU

SER

ILE

THR

ARG

THR

SER

ARG

ALA

PRO

3

SER

VAL

ALA

THR

VAL

GLY

SER

ILE

CYS

ASP

4

LEU

ASN

LEU

LYS

ILE

PRO

GLU

ILE

ASN

SER

5

SER

ASP

MET

SER

ALA

HIS

VAL

THR

SER

PRO

6

SER

GLY

ARG

VAL

THR

GLU

ALA

GLU

ILE

VAL

7

PRO

MET

GLY

LYS

ASN

SER

HIS

CYS

VAL

ARG

8

PHE

VAL

PRO

GLN

GLU

MET

GLY

VAL

HIS

THR

9

VAL

SER

VAL

LYS

TYR

ARG

GLY

GLN

HIS

VAL

10

THR

GLY

SER

PRO

PHE

GLN

PHE

THR

VAL

GLY

11

PRO

LEU

GLY

GLU

GLY

Samples:

sample_1: filamin domain, [U-13C; U-15N], 1.53 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	not available	condition_1
3D 15N-separated NOESY	sample_1	not available	condition_1

Software:

TOPSPIN v1.3, Bruker - collection

NMRPipe v20060524, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9747, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE II 900 MHz

PDB	2E9I
GB	AAL68442 AAL68443
REF	XP_006040270 XP_006040272