BMRB Entry 10212
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10212
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Title: Solution structure of the SH3 domain of the human SRC-like adopter protein (SLAP)
Deposition date: 2008-04-09 Original release date: 2010-03-11
Authors: Ohnishi, S.; Kigawa, T.; Tochio, N.; Sato, M.; Nameki, N.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation: Ohnishi, S.; Kigawa, T.; Tochio, N.; Sato, M.; Nameki, N.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the SH3 domain of the human SRC-like adopter protein (SLAP)" .
Assembly members:
SH3 domain, polymer, 79 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: Cell free synthesis Vector: P040921-09
Entity Sequences (FASTA):
SH3 domain: GSSGSSGPLPNPEGLDSDFL
AVLSDYPSPDISPPIFRRGE
KLRVISDEGGWWKAISLSTG
RESYIPGICVARVSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 304 |
| 15N chemical shifts | 68 |
| 1H chemical shifts | 475 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SH3 domain | 1 |
Entities:
Entity 1, SH3 domain 79 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | PRO | LEU | PRO | ||||
| 2 | ASN | PRO | GLU | GLY | LEU | ASP | SER | ASP | PHE | LEU | ||||
| 3 | ALA | VAL | LEU | SER | ASP | TYR | PRO | SER | PRO | ASP | ||||
| 4 | ILE | SER | PRO | PRO | ILE | PHE | ARG | ARG | GLY | GLU | ||||
| 5 | LYS | LEU | ARG | VAL | ILE | SER | ASP | GLU | GLY | GLY | ||||
| 6 | TRP | TRP | LYS | ALA | ILE | SER | LEU | SER | THR | GLY | ||||
| 7 | ARG | GLU | SER | TYR | ILE | PRO | GLY | ILE | CYS | VAL | ||||
| 8 | ALA | ARG | VAL | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: SH3 domain, [U-13C; U-15N], 1.28 mM; d-TrisHCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | not available | condition_1 |
| 3D 13C-separated NOESY | sample_1 | not available | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0,4, Johnson, B. A. - data analysis
Kujira v0.9295, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 900 MHz
Related Database Links:
| PDB | |
| DBJ | BAA13758 BAE89471 BAG35478 BAG73760 BAH12578 |
| EMBL | CAB53536 CAG38774 |
| GB | AAC27662 AAC50357 AAH07042 AAV38795 AAV38796 |
| PRF | 2204265A |
| REF | NP_001039021 NP_001039022 NP_001269893 NP_001274231 NP_006739 |
| SP | Q13239 |
| AlphaFold | Q13239 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts