BMRB Entry 10273
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR10273
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structures of the TGS domain of human developmentally-regulated GTP-binding protein 1
Deposition date: 2008-12-15 Original release date: 2009-12-14
Authors: Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.
Citation: Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structures of the TGS domain of human developmentally-regulated GTP-binding protein 1" .
Assembly members:
TGS domain, polymer, 93 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Vector: P051011-16
Entity Sequences (FASTA):
TGS domain: GSSGSSGYLKLVRIYTKPKG
QLPDYTSPVVLPYSRTTVED
FCMKIHKNLIKEFKYALVWG
LSVKHNPQKVGKDHTLEDED
VIQIVKKSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 406 |
| 15N chemical shifts | 81 |
| 1H chemical shifts | 634 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TGS domain | 1 |
Entities:
Entity 1, TGS domain 93 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | TYR | LEU | LYS | ||||
| 2 | LEU | VAL | ARG | ILE | TYR | THR | LYS | PRO | LYS | GLY | ||||
| 3 | GLN | LEU | PRO | ASP | TYR | THR | SER | PRO | VAL | VAL | ||||
| 4 | LEU | PRO | TYR | SER | ARG | THR | THR | VAL | GLU | ASP | ||||
| 5 | PHE | CYS | MET | LYS | ILE | HIS | LYS | ASN | LEU | ILE | ||||
| 6 | LYS | GLU | PHE | LYS | TYR | ALA | LEU | VAL | TRP | GLY | ||||
| 7 | LEU | SER | VAL | LYS | HIS | ASN | PRO | GLN | LYS | VAL | ||||
| 8 | GLY | LYS | ASP | HIS | THR | LEU | GLU | ASP | GLU | ASP | ||||
| 9 | VAL | ILE | GLN | ILE | VAL | LYS | LYS | SER | GLY | PRO | ||||
| 10 | SER | SER | GLY |
Samples:
sample_1: TGS domain, [U-13C; U-15N], 1 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9827, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 700 MHz
Related Database Links:
| PDB | |
| DBJ | BAA01555 BAC38030 BAE25890 BAE33021 BAG38025 |
| EMBL | CAA06775 CAB61403 CAG30374 CAG46856 CAK54479 |
| GB | AAD12240 AAH19285 AAH20803 AAH39649 AAH88410 |
| REF | NP_001009685 NP_001029863 NP_001162241 NP_001164823 NP_001253866 |
| SP | P32233 Q3MHP5 Q9Y295 |
| TPG | DAA20497 DAA22636 |
| AlphaFold | Q3MHP5 P32233 Q9Y295 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts