BMRB Entry 10308
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10308
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Title: Solution structure of the C2H2 type zinc finger (region 484-512) of human Zinc finger protein 473
Deposition date: 2009-03-10 Original release date: 2010-03-11
Authors: Tomizawa, T.; Tochio, N.; Abe, H.; Saito, K.; Li, H.; Sato, M.; Koshiba, S.; Kobayashi, N.; Kigawa, T.; Yokoyama, S.
Citation: Tochio, N.; Tomizawa, T.; Abe, H.; Saito, K.; Li, H.; Sato, M.; Koshiba, S.; Kobayashi, N.; Kigawa, T.; Yokoyama, S.. "Solution structure of the C2H2 type zinc finger (region 484-512) of human Zinc finger protein 473" .
Assembly members:
zf-C2H2, polymer, 42 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell-free synthesis Vector: P070207-06
Entity Sequences (FASTA):
zf-C2H2: GSSGSSGEKPYSCAECKETF
SDNNRLVQHQKMHTVKSGPS
SG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 152 |
| 15N chemical shifts | 36 |
| 1H chemical shifts | 228 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | zf-C2H2 | 1 |
| 2 | ZINC ION | 2 |
Entities:
Entity 1, zf-C2H2 42 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | GLU | LYS | PRO | ||||
| 2 | TYR | SER | CYS | ALA | GLU | CYS | LYS | GLU | THR | PHE | ||||
| 3 | SER | ASP | ASN | ASN | ARG | LEU | VAL | GLN | HIS | GLN | ||||
| 4 | LYS | MET | HIS | THR | VAL | LYS | SER | GLY | PRO | SER | ||||
| 5 | SER | GLY |
Entity 2, ZINC ION - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: zf-C2H2, [U-13C; U-15N], 1.0 ± 0.1 mM; d-Tris-HCl 20 mM; NaCl 100 mM; ZnCl2 0.05 mM; IDA 1 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
TOPSPIN v1.3, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9820, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE II 900 MHz
Related Database Links:
| PDB |
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