Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11052
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Citation: Sakamoto, Keiko; Hirai, Ken-ichi; Kitamura, Yoshiaki; Yamazaki, Kouta; Yusa, Mitsunobu; Tokunaga, Naoki; Doi, Gakuji; Noda, Yasuo; Tachibana, Hideki; Segawa, Shin-Ichi. "Glycerol-induced folding of unstructured disulfide-deficient lysozyme into a
native-like conformation" Biopolymers 91, 665-675 (2009).
PubMed: 19353641
Assembly members:
2SS(6-127_64-80), polymer, 130 residues, Formula weight is not available
Natural source: Common Name: chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pYK1
Entity Sequences (FASTA):
2SS(6-127_64-80): MKVFGRCELAAAMKRHGLDN
YRGYSLGNWVAAAKFESNFN
TQATNRNTDGSTDYGILQIN
SRWWCNDGRTPGSRNLANIP
CSALLSSDITASVNAAKKIV
SDGNGMNAWVAWRNRAKGTD
VQAWIRGCRL
Data type | Count |
15N chemical shifts | 123 |
1H chemical shifts | 517 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | 2SS(6-127_64-80) | 1 |
Entity 1, 2SS(6-127_64-80) 130 residues - Formula weight is not available
1 | MET | LYS | VAL | PHE | GLY | ARG | CYS | GLU | LEU | ALA | |
2 | ALA | ALA | MET | LYS | ARG | HIS | GLY | LEU | ASP | ASN | |
3 | TYR | ARG | GLY | TYR | SER | LEU | GLY | ASN | TRP | VAL | |
4 | ALA | ALA | ALA | LYS | PHE | GLU | SER | ASN | PHE | ASN | |
5 | THR | GLN | ALA | THR | ASN | ARG | ASN | THR | ASP | GLY | |
6 | SER | THR | ASP | TYR | GLY | ILE | LEU | GLN | ILE | ASN | |
7 | SER | ARG | TRP | TRP | CYS | ASN | ASP | GLY | ARG | THR | |
8 | PRO | GLY | SER | ARG | ASN | LEU | ALA | ASN | ILE | PRO | |
9 | CYS | SER | ALA | LEU | LEU | SER | SER | ASP | ILE | THR | |
10 | ALA | SER | VAL | ASN | ALA | ALA | LYS | LYS | ILE | VAL | |
11 | SER | ASP | GLY | ASN | GLY | MET | ASN | ALA | TRP | VAL | |
12 | ALA | TRP | ARG | ASN | ARG | ALA | LYS | GLY | THR | ASP | |
13 | VAL | GLN | ALA | TRP | ILE | ARG | GLY | CYS | ARG | LEU |
sample_1: 2SS(6-127_64-80), [U-99% 15N], 1 mM; dichloroacetic acid, [U-2H], 60 mM; DMSO-d6, [U-2H], 95%; H2O 5%
sample_2: 2SS(6-127_64-80), [U-99% 15N], 0.2 mM; dichloroacetic acid, [U-2H], 60 mM; DMSO-d6, [U-2H], 95%; H2O 5%
sample_conditions_1: pH*: 5.5; pressure: 1 atm; temperature: 298 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N HSQC-NOESY-HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
xwinnmr v3.5, Bruker Biospin - collection, processing
SPARKY v3.113, Goddard - chemical shift assignment, peak picking
Download HSQC peak lists in one of the following formats:
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