BMRB Entry 11056
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11056
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Vermeer, Louic; Reat, Valerie; Hemminga, Marcus; Milon, Alain. "Structural properties of a peptide derived from H(+)-V-ATPase subunit a" Biochim. Biophys. Acta. 1788, 1204-1212 (2009).
PubMed: 19249284
Assembly members:
KMTM7, polymer, 25 residues, 2783 Da.
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
KMTM7: KKSHTASYLRLWALSLAHAQ
LSSKK
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 1H chemical shifts | 173 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | KMTM7 | 1 |
Entities:
Entity 1, KMTM7 25 residues - 2783 Da.
the 2 lysine residues on both the N-term and C-term are non-native
| 1 | LYS | LYS | SER | HIS | THR | ALA | SER | TYR | LEU | ARG | ||||
| 2 | LEU | TRP | ALA | LEU | SER | LEU | ALA | HIS | ALA | GLN | ||||
| 3 | LEU | SER | SER | LYS | LYS |
Samples:
sample_1: KMTM7 1 mM; SDS, [U-100% 2H], 250 mM; sodium phosphate 10 mM; DSS 0.3 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 5.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
ARIA v2.2, Linge, O'Donoghue and Nilges - structure solution
AQUA v3.2, Rullmann, Doreleijers and Kaptein - data analysis
SPARKY, Goddard - chemical shift assignment, peak picking
ProcheckNMR, Laskowski and MacArthur - data analysis
TOPSPIN, Bruker Biospin - collection
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| BMRB | 15025 |
| PDB | |
| DBJ | BAB62291 BAB62292 BAB62293 GAA25496 GAA26581 |
| EMBL | CAA20334 CAA61776 CAA77448 CAA77453 CAA89764 |
| GB | AAA35211 AAD34771 AAF28475 AAF53116 AAF55550 |
| REF | NP_001023017 NP_001023018 NP_001023019 NP_001023020 NP_001023021 |
| SP | P30628 P32563 P37296 |
| TPG | DAA09953 DAA11036 |
| AlphaFold | P30628 P32563 P37296 |