BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11098

Title: Solution structure of the sixth fibronectin type III domain of human Netrin receptor DCC

Deposition date: 2010-02-19 Original release date: 2011-02-17

Authors: Tochio, N.; Koshiba, S.; Tomizawa, T.; Yoneyama, M.; Kigawa, T.; Yokoyama, S.

Citation: Tochio, N.; Koshiba, S.; Tomizawa, T.; Yoneyama, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the sixth fibronectin type III domain of human Netrin receptor DCC"  .

Assembly members:
sixth fn3 domain, polymer, 114 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050905-24

Entity Sequences (FASTA):
sixth fn3 domain: GSSGSSGPTSAPKDLTVITR EGKPRAVIVSWQPPLEANGK ITAYILFYTLDKNIPIDDWI METISGDRLTHQIMDLNLDT MYYFRIQARNSKGVGPLSDP ILFRTLKVSGPSSG

Data sets:
Data typeCount
13C chemical shifts501
15N chemical shifts109
1H chemical shifts784

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1sixth fn3 domain1

Entities:

Entity 1, sixth fn3 domain 114 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYPROTHRSER
2   ALAPROLYSASPLEUTHRVALILETHRARG
3   GLUGLYLYSPROARGALAVALILEVALSER
4   TRPGLNPROPROLEUGLUALAASNGLYLYS
5   ILETHRALATYRILELEUPHETYRTHRLEU
6   ASPLYSASNILEPROILEASPASPTRPILE
7   METGLUTHRILESERGLYASPARGLEUTHR
8   HISGLNILEMETASPLEUASNLEUASPTHR
9   METTYRTYRPHEARGILEGLNALAARGASN
10   SERLYSGLYVALGLYPROLEUSERASPPRO
11   ILELEUPHEARGTHRLEULYSVALSERGLY
12   PROSERSERGLY

Samples:

sample_1: sixth fn3 domain, [U-13C; U-15N], 1.3 mM; d-Tris-HCl, [U-2H], 20 mM; sodium chloride 100 mM; d-DTT, [U-2H], 1 mM; sodium azide 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropiccondition_1
3D 1H-13C NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMNMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.955, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts