BMRB Entry 11217
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11217
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structure of the first SH3 domain of Stac protein
Deposition date: 2010-07-22 Original release date: 2011-07-21
Authors: Qin, X.; Suetake, T.; Hayashi, F.; Yokoyama, S.
Citation: Qin, X.; Suetake, T.; Hayashi, F.; Yokoyama, S.. "Solution structure of the first SH3 domain of Stac protein" .
Assembly members:
SH3 domain, polymer, 68 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050613-10
Entity Sequences (FASTA):
SH3 domain: GSSGSSGNTYVALYKFVPQE
NEDLEMRPGDIITLLEDSNE
DWWKGKIQDRIGFFPANFVQ
RLSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 289 |
| 15N chemical shifts | 68 |
| 1H chemical shifts | 447 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SH3 domain of Stac protein | 1 |
Entities:
Entity 1, SH3 domain of Stac protein 68 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ASN | THR | TYR | ||||
| 2 | VAL | ALA | LEU | TYR | LYS | PHE | VAL | PRO | GLN | GLU | ||||
| 3 | ASN | GLU | ASP | LEU | GLU | MET | ARG | PRO | GLY | ASP | ||||
| 4 | ILE | ILE | THR | LEU | LEU | GLU | ASP | SER | ASN | GLU | ||||
| 5 | ASP | TRP | TRP | LYS | GLY | LYS | ILE | GLN | ASP | ARG | ||||
| 6 | ILE | GLY | PHE | PHE | PRO | ALA | ASN | PHE | VAL | GLN | ||||
| 7 | ARG | LEU | SER | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: SH3 domain, [U-13C; U-15N], 1.09 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
VNMR v6.1C, Varian - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9296, Kobayashi N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Varian INOVA 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAA13152 BAG36275 BAG61208 BAG63925 BAI47349 |
| GB | AAH20221 ABM83106 ABM86300 ABM86301 AIC55179 |
| REF | NP_001278978 NP_003140 XP_001101503 XP_002803096 XP_003826166 |
| SP | Q99469 |
| AlphaFold | Q99469 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts