BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11248

Title: Solution structure of the PDZ domain from mouse LIM domain kinase

Deposition date: 2010-07-23 Original release date: 2011-08-03

Authors: Hayashi, F.; Suetake, T.; Yokoyama, S.

Citation: Hayashi, F.; Suetake, T.; Yokoyama, S.. "Solution structure of the PDZ domain from mouse LIM domain kinase"  .

Assembly members:
PDZ domain, polymer, 118 residues, Formula weight is not available

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P041220-03

Entity Sequences (FASTA):
PDZ domain: GSSGSSGVQDQLPYSVTLIS MPATTECRRGFSVTVESASS NYATTVQVKEVNRMHISPNN RNAIHPGDRILEINGTPVRT LRVEEVEDAIKQTSQTLQLL IEHDPVPQRLDQSGPSSG

Data sets:
Data typeCount
13C chemical shifts483
15N chemical shifts115
1H chemical shifts782

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PDZ domain1

Entities:

Entity 1, PDZ domain 118 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYVALGLNASP
2   GLNLEUPROTYRSERVALTHRLEUILESER
3   METPROALATHRTHRGLUCYSARGARGGLY
4   PHESERVALTHRVALGLUSERALASERSER
5   ASNTYRALATHRTHRVALGLNVALLYSGLU
6   VALASNARGMETHISILESERPROASNASN
7   ARGASNALAILEHISPROGLYASPARGILE
8   LEUGLUILEASNGLYTHRPROVALARGTHR
9   LEUARGVALGLUGLUVALGLUASPALAILE
10   LYSGLNTHRSERGLNTHRLEUGLNLEULEU
11   ILEGLUHISASPPROVALPROGLNARGLEU
12   ASPGLNSERGLYPROSERSERGLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 0.47 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.1, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAA06675 BAA24488 BAA24489 BAA24491 BAA29035
GB AAC39947 AAH07129 ABK42255 EDL40412 EDL40413
REF NP_001029202 NP_034848 NP_774958 XP_005366157 XP_006514610
SP O54785
AlphaFold O54785

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts