BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11272

Title: Solution Structure of the Novel Identified Ubiquitin-like Domain in the Human COBL-like 1 Protein

Deposition date: 2010-08-09 Original release date: 2011-08-18

Authors: Zhao, C.; Kigawa, T.; Saito, K.; Koshiba, S.; Inoue, M.; Yokoyama, S.

Citation: Zhao, C.; Kigawa, T.; Saito, K.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution Structure of the Novel Identified Ubiquitin-like Domain in the Human COBL-like 1 Protein"  .

Assembly members:
ubiquitin-like domain, polymer, 91 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P050719-08

Entity Sequences (FASTA):
ubiquitin-like domain: GSSGSSGEKTVRVVINFKKT QKTIVRVSPHASLQELAPII CSKCEFDPLHTLLLKDYQSQ EPLDLTKSLNDLGLRELYAM DVNRESGPSSG

Data sets:
Data typeCount
13C chemical shifts384
15N chemical shifts83
1H chemical shifts613

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ubiquitin-like domain1

Entities:

Entity 1, ubiquitin-like domain 91 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLULYSTHR
2   VALARGVALVALILEASNPHELYSLYSTHR
3   GLNLYSTHRILEVALARGVALSERPROHIS
4   ALASERLEUGLNGLULEUALAPROILEILE
5   CYSSERLYSCYSGLUPHEASPPROLEUHIS
6   THRLEULEULEULYSASPTYRGLNSERGLN
7   GLUPROLEUASPLEUTHRLYSSERLEUASN
8   ASPLEUGLYLEUARGGLULEUTYRALAMET
9   ASPVALASNARGGLUSERGLYPROSERSER
10   GLY

Samples:

sample_1: ubiquitin-like domain, [U-13C; U-15N], 1.14 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298.0 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9321, Kobayashi, N. - data analysis

CYANA v1.0.8, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAA76821 BAG09927 BAH11931
EMBL CAH89676
GB AAH71588 AAX93068 EAX11339 EAX11340 EFB27997
REF NP_001265387 NP_001265389 NP_001265390 NP_055715 XP_001098489
SP Q53SF7
AlphaFold Q53SF7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts