BMRB Entry 11302
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11302
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Title: Solution structure of the N-terminal C2H2 type zinc-binding domain of the Zinc finger protein 64, isoforms 1 and 2
Deposition date: 2010-08-09 Original release date: 2011-08-19
Authors: Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the N-terminal C2H2 type zinc-binding domain of the Zinc finger protein 64, isoforms 1 and 2" .
Assembly members:
C2H2-type zinc-binding domain, polymer, 96 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P050815-12
Entity Sequences (FASTA):
C2H2-type zinc-binding domain: GSSGSSGPHKCEVCGKCFSR
KDKLKTHMRCHTGVKPYKCK
TCDYAAADSSSLNKHLRIHS
DERPFKCQICPYASRNSSQL
TVHLRSHTGDSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 373 |
| 15N chemical shifts | 75 |
| 1H chemical shifts | 561 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | C2H2-type zinc-binding domain | 1 |
| 2 | ZINC ION no.1 | 2 |
| 3 | ZINC ION no.2 | 2 |
| 4 | ZINC ION no.3 | 2 |
Entities:
Entity 1, C2H2-type zinc-binding domain 96 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | PRO | HIS | LYS | ||||
| 2 | CYS | GLU | VAL | CYS | GLY | LYS | CYS | PHE | SER | ARG | ||||
| 3 | LYS | ASP | LYS | LEU | LYS | THR | HIS | MET | ARG | CYS | ||||
| 4 | HIS | THR | GLY | VAL | LYS | PRO | TYR | LYS | CYS | LYS | ||||
| 5 | THR | CYS | ASP | TYR | ALA | ALA | ALA | ASP | SER | SER | ||||
| 6 | SER | LEU | ASN | LYS | HIS | LEU | ARG | ILE | HIS | SER | ||||
| 7 | ASP | GLU | ARG | PRO | PHE | LYS | CYS | GLN | ILE | CYS | ||||
| 8 | PRO | TYR | ALA | SER | ARG | ASN | SER | SER | GLN | LEU | ||||
| 9 | THR | VAL | HIS | LEU | ARG | SER | HIS | THR | GLY | ASP | ||||
| 10 | SER | GLY | PRO | SER | SER | GLY |
Entity 2, ZINC ION no.1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: C2H2 type zinc-binding domain, [U-13C; U-15N], 1.28 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 50 uM; IDA 1 mM; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.9736, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts