BMRB Entry 11308

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PDB ID: 1wir
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11308
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the C2H2 zinc finger domain of the protein arginine N-methyltransferase 3 from Mus musculus

Deposition date:

2010-08-10

Original release date:

2011-08-19

Authors:

Miyamoto, K.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Miyamoto, K.; Tomizawa, T.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the C2H2 zinc finger domain of the protein arginine N-methyltransferase 3 from Mus musculus" .

Assembly members:

Assembly members:
C2H2 zinc finger domain, polymer, 121 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: house mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P040126-40

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C2H2 zinc finger domain: GSSGSSGEPAHGRQHTPCLF CDRLFASAEETFSHCKLEHQ FNIDSMVHKHGLEFYGYIKL INFIRLKNPTVEYMNSIYNP VPWEKDEYLKPVLEDDLLLQ FDVEDLYEPVSTPFSSGPSS G

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	542
15N chemical shifts	112
1H chemical shifts	809

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C2H2 zinc finger domain	1
2	ZINC ION	2

Entities:

Entity 1, C2H2 zinc finger domain 121 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

GLU

PRO

ALA

2

HIS

GLY

ARG

GLN

HIS

THR

PRO

CYS

LEU

PHE

3

CYS

ASP

ARG

LEU

PHE

ALA

SER

ALA

GLU

4

THR

PHE

SER

HIS

CYS

LYS

LEU

GLU

HIS

GLN

5

PHE

ASN

ILE

ASP

SER

MET

VAL

HIS

LYS

HIS

6

GLY

LEU

GLU

PHE

TYR

GLY

TYR

ILE

LYS

LEU

7

ILE

ASN

PHE

ILE

ARG

LEU

LYS

ASN

PRO

THR

8

VAL

GLU

TYR

MET

ASN

SER

ILE

TYR

ASN

PRO

9

VAL

PRO

TRP

GLU

LYS

ASP

GLU

TYR

LEU

LYS

10

PRO

VAL

LEU

GLU

ASP

LEU

GLN

11

PHE

ASP

VAL

GLU

ASP

LEU

TYR

GLU

PRO

VAL

12

SER

THR

PRO

PHE

SER

GLY

PRO

SER

13

GLY

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: C2H2 zinc finger domain, [U-13C; U-15N], 1.14 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.8996, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	1WIR
DBJ	BAC25300 BAC27531 BAC39708 BAG60456
GB	AAH50775 AAN84530 EAW68334 EDL22978 ERE78768
REF	NP_001138639 NP_598501 XP_003254364 XP_004711656 XP_005578451
SP	Q922H1
AlphaFold	Q922H1