BMRB Entry 11312
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11312
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Title: Solution structure of the C2H2 type zinc-binding domain of human zinc finger protein 64, isoforms 1 and 2
Deposition date: 2010-08-10 Original release date: 2011-08-19
Authors: Yoneyama, M.; Koshiba, S.; Tochio, N.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation: Yoneyama, M.; Koshiba, S.; Tochio, N.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the C2H2 type zinc-binding domain of human zinc finger protein 64, isoforms 1 and 2" .
Assembly members:
C2H2 type zinc-binding domain, polymer, 70 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P040809-09
Entity Sequences (FASTA):
C2H2 type zinc-binding domain: GSSGSSGHPEKCSECSYSCS
SKAALRIHERIHCTDRPFKC
NYCSFDTKQPSNLSKHMKKF
HGDMSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 249 |
| 15N chemical shifts | 51 |
| 1H chemical shifts | 372 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | C2H2 type zinc-binding domain | 1 |
| 2 | ZINC ION no.1 | 2 |
| 3 | ZINC ION no.2 | 2 |
Entities:
Entity 1, C2H2 type zinc-binding domain 70 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | HIS | PRO | GLU | |
| 2 | LYS | CYS | SER | GLU | CYS | SER | TYR | SER | CYS | SER | |
| 3 | SER | LYS | ALA | ALA | LEU | ARG | ILE | HIS | GLU | ARG | |
| 4 | ILE | HIS | CYS | THR | ASP | ARG | PRO | PHE | LYS | CYS | |
| 5 | ASN | TYR | CYS | SER | PHE | ASP | THR | LYS | GLN | PRO | |
| 6 | SER | ASN | LEU | SER | LYS | HIS | MET | LYS | LYS | PHE | |
| 7 | HIS | GLY | ASP | MET | SER | GLY | PRO | SER | SER | GLY |
Entity 2, ZINC ION no.1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: C2H2 type zinc-binding domain, [U-13C; U-15N], 1.25 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 50 uM; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20031121, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.921, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 700 MHz
Related Database Links:
| PDB |
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