BMRB Entry 11322
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11322
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NMR-STAR v3 text file.
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Title: Solution structure of the SANT domain of human KIAA1915 protein PubMed: 17428495
Deposition date: 2010-08-10 Original release date: 2010-12-01
Authors: Yoneyama, M.; Umehara, T.; Saito, K.; Tochio, N.; Koshiba, S.; Inoue, M.; Tanaka, A.; Kigawa, T.; Yokoyama, S.
Citation: Yoneyama, Misao; Tochio, Naoya; Umehara, Takashi; Koshiba, Seizo; Inoue, Makoto; Yabuki, Takashi; Aoki, Masaaki; Seki, Eiko; Matsuda, Takayoshi; Watanabe, Satoru; Tomo, Yasuko; Nishimura, Yuji; Harada, Takushi; Terada, Takaho; Shirouzu, Mikako; Hayashizaki, Yoshihide; Ohara, Osamu; Tanaka, Akiko; Kigawa, Takanori; Yokoyama, Shigeyuki. "Structural and functional differences of SWIRM domain subtypes." J. Mol. Biol. 369, 222-238 (2007).
Assembly members:
SANT domain, polymer, 72 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P041018-13
Entity Sequences (FASTA):
SANT domain: GSSGSSGYSVKWTIEEKELF
EQGLAKFGRRWTKISKLIGS
RTVLQVKSYARQYFKNKVKC
GLDKETPNQKTG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 329 |
| 15N chemical shifts | 80 |
| 1H chemical shifts | 519 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SANT domain | 1 |
Entities:
Entity 1, SANT domain 72 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | TYR | SER | VAL | ||||
| 2 | LYS | TRP | THR | ILE | GLU | GLU | LYS | GLU | LEU | PHE | ||||
| 3 | GLU | GLN | GLY | LEU | ALA | LYS | PHE | GLY | ARG | ARG | ||||
| 4 | TRP | THR | LYS | ILE | SER | LYS | LEU | ILE | GLY | SER | ||||
| 5 | ARG | THR | VAL | LEU | GLN | VAL | LYS | SER | TYR | ALA | ||||
| 6 | ARG | GLN | TYR | PHE | LYS | ASN | LYS | VAL | LYS | CYS | ||||
| 7 | GLY | LEU | ASP | LYS | GLU | THR | PRO | ASN | GLN | LYS | ||||
| 8 | THR | GLY |
Samples:
sample_1: SANT domain, [U-13C; U-15N], 0.83 mM; Acetate buffer Na 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 4.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v2.6, Bruker - collection
NMRPipe v20020425, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B. A. - data analysis
Kujira v0.9295, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAB67808 |
| GB | AAI67849 |
| REF | NP_001078956 XP_003824269 XP_004025928 XP_006710377 XP_008976222 |
| SP | Q5VVJ2 |
| AlphaFold | Q5VVJ2 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
or all simulated shifts