BMRB Entry 11345

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PDB ID: 2elj
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11345
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the SWIRM domain of baker's yeast Transcriptional adapter 2

Deposition date:

2010-08-10

Original release date:

2011-08-19

Authors:

Yoneyama, M.; Tochio, N.; Koshiba, S.; Tomizawa, T.; Watanabe, S.; Harada, T.; Umehara, T.; Tanaka, A.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Yoneyama, M.; Tochio, N.; Koshiba, S.; Tomizawa, T.; Watanabe, S.; Harada, T.; Umehara, T.; Tanaka, A.; Kigawa, T.; Yokoyama, S.. "Solution structure of the SWIRM domain of baker's yeast Transcriptional adapter 2" .

Assembly members:

Assembly members:
SWIRM, polymer, 88 residues, Formula weight is not available

Natural source:

Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P060216-01

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SWIRM: GSSGSSGNMTISDIQHAPDY ALLSNDEQQLCIQLKILPKP YLVLKEVMFRELLKTGGNLS KSACRELLNIDPIKANRIYD FFQSQNWM

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	382
15N chemical shifts	87
1H chemical shifts	627

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SWIRM	1

Entities:

Entity 1, SWIRM 88 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ASN

MET

THR

2

ILE

SER

ASP

ILE

GLN

HIS

ALA

PRO

ASP

TYR

3

ALA

LEU

SER

ASN

ASP

GLU

GLN

LEU

4

CYS

ILE

GLN

LEU

LYS

ILE

LEU

PRO

LYS

PRO

5

TYR

LEU

VAL

LEU

LYS

GLU

VAL

MET

PHE

ARG

6

GLU

LEU

LYS

THR

GLY

ASN

LEU

SER

7

LYS

SER

ALA

CYS

ARG

GLU

LEU

ASN

ILE

8

ASP

PRO

ILE

LYS

ALA

ASN

ARG

ILE

TYR

ASP

9

PHE

GLN

SER

GLN

ASN

TRP

MET

Samples:

sample_1: SWIRM, [U-13C; U-15N], 0.3 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

TOPSPIN v1.3, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE2 900 MHz