BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11399

Title: solution structure of RNA binding domain in PTB-like protein L

Deposition date: 2010-09-09 Original release date: 2011-09-08

Authors: Tsuda, K.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.

Citation: Tsuda, K.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.. "solution structure of RNA binding domain in PTB-like protein L"  .

Assembly members:
RNA recognition motif, polymer, 101 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P040921-04

Entity Sequences (FASTA):
RNA recognition motif: GSSGSSGDKMDGAPSRVLHI RKLPGEVTETEVIALGLPFG KVTNILMLKGKNQAFLELAT EEAAITMVNYYSAVTPHLRN QPIYIQYSNHKELKTSGPSS G

Data sets:
Data typeCount
13C chemical shifts413
15N chemical shifts87
1H chemical shifts663

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RNA recognition motif1

Entities:

Entity 1, RNA recognition motif 101 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYASPLYSMET
2   ASPGLYALAPROSERARGVALLEUHISILE
3   ARGLYSLEUPROGLYGLUVALTHRGLUTHR
4   GLUVALILEALALEUGLYLEUPROPHEGLY
5   LYSVALTHRASNILELEUMETLEULYSGLY
6   LYSASNGLNALAPHELEUGLULEUALATHR
7   GLUGLUALAALAILETHRMETVALASNTYR
8   TYRSERALAVALTHRPROHISLEUARGASN
9   GLNPROILETYRILEGLNTYRSERASNHIS
10   LYSGLULEULYSTHRSERGLYPROSERSER
11   GLY

Samples:

sample_1: RNA recognition motif, [U-13C; U-15N], 1.34 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9297, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAB71742 BAB71743 BAD92503 BAE20599 BAE20681
EMBL CAB54073
GB AAF14284 AAF21807 AAH10255 AAH16582 AAH82076
PIR JC7526
REF NP_001005555 NP_001103551 NP_001287914 NP_001287915 NP_001287916
SP Q66H20 Q91Z31 Q9UKA9
TPG DAA00060 DAA00061 DAA31417
AlphaFold Q9UKA9 Q91Z31 Q66H20

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts