BMRB Entry 11413
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11413
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Refinement of RNA binding domain in human Tra2 beta protein
Deposition date: 2010-09-09 Original release date: 2011-09-08
Authors: Tsuda, K.; Kuwasako, K.; Takahashi, M.; Someya, T.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Sugano, S.; Muto, Y.; Yokoyama, S.
Citation: Tsuda, K.; Kuwasako, K.; Someya, T.; Takahashi, M.; He, F.; Inoue, M.; Harada, T.; Watanabe, S.; Terada, T.; Kobayashi, N.; Shirouzu, M.; Kigawa, T.; Tanaka, A.; Sugano, S.; Guntert, P.; Yokoyama, S.; Muto, Y.. "Refinement of RNA binding domain in human Tra2 beta protein" .
Assembly members:
RNA recognition motif, polymer, 96 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free
Entity Sequences (FASTA):
RNA recognition motif: GPLGSRANPDPNCCLGVFGL
SLYTTERDLREVFSKYGPIA
DVSIVYDQQSRRSRGFAFVY
FENVDDAKEAKERANGMELD
GRRIRVDFSITKRPHT
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 333 |
| 15N chemical shifts | 96 |
| 1H chemical shifts | 650 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RNA recognition motif | 1 |
Entities:
Entity 1, RNA recognition motif 96 residues - Formula weight is not available
| 1 | GLY | PRO | LEU | GLY | SER | ARG | ALA | ASN | PRO | ASP | ||||
| 2 | PRO | ASN | CYS | CYS | LEU | GLY | VAL | PHE | GLY | LEU | ||||
| 3 | SER | LEU | TYR | THR | THR | GLU | ARG | ASP | LEU | ARG | ||||
| 4 | GLU | VAL | PHE | SER | LYS | TYR | GLY | PRO | ILE | ALA | ||||
| 5 | ASP | VAL | SER | ILE | VAL | TYR | ASP | GLN | GLN | SER | ||||
| 6 | ARG | ARG | SER | ARG | GLY | PHE | ALA | PHE | VAL | TYR | ||||
| 7 | PHE | GLU | ASN | VAL | ASP | ASP | ALA | LYS | GLU | ALA | ||||
| 8 | LYS | GLU | ARG | ALA | ASN | GLY | MET | GLU | LEU | ASP | ||||
| 9 | GLY | ARG | ARG | ILE | ARG | VAL | ASP | PHE | SER | ILE | ||||
| 10 | THR | LYS | ARG | PRO | HIS | THR |
Samples:
sample_1: Human transformer 2 beta-1, [U-13C; U-15N], 1 mM; H2O 90%; D2O 10%
condition_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY | sample_1 | isotropic | condition_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | condition_1 |
Software:
AMBER v9, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, Kollm - refinement
xwinnmr v3.6, Bruker, Biospin - collection
NMRPipe v20060801, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer, and, Bax - processing
NMRView v5.0.4, Johnson, One, Moon, Scientific - data analysis
Kujira v0.9843, N. Kobayashi - data analysis
CYANA v2.1, Guntert, Mumenthaler, and, Wuthrich - structure solution
NMR spectrometers:
- Bruker Avance 800 MHz
Related Database Links:
| BMRB | 16920 |
| PDB | |
| DBJ | BAA08556 BAC05256 BAC33819 BAD92445 BAE88784 |
| EMBL | CAA56518 CAH18071 |
| GB | AAB08701 AAC28242 AAD19277 AAD19278 AAH00160 |
| REF | NP_001029948 NP_001070689 NP_001230808 NP_001248265 NP_001271939 |
| SP | P62995 P62996 P62997 Q3ZBT6 |
| TPG | DAA33375 |
| AlphaFold | P62996 P62995 Q3ZBT6 P62997 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts