BMRB Entry 11453
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11453
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Title: Backbone chemical shifts assignment of UHRF1 atypical PHD finger PubMed: 22100450
Deposition date: 2011-09-22 Original release date: 2017-03-14
Authors: Qian, Chengmin
Citation: Xie, Si; Jakoncic, Jean; Qian, Chengmin. "UHRF1 double tudor domain and the adjacent PHD finger act together to recognize K9me3-containing histone H3 tail" J. Mol. Biol. 415, 318-328 (2012).
Assembly members:
PHD_finger, polymer, 74 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX4T1
Entity Sequences (FASTA):
PHD_finger: GSPEFGPSCKHCKDDVNRLC
RVCACHLCGGRQDPDKQLMC
DECDMAFHIYCLDPPLSSVP
SEDEWYCPECRNDA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 122 |
| 15N chemical shifts | 64 |
| 1H chemical shifts | 64 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PHD finger | 1 |
Entities:
Entity 1, PHD finger 74 residues - Formula weight is not available
| 1 | GLY | SER | PRO | GLU | PHE | GLY | PRO | SER | CYS | LYS | ||||
| 2 | HIS | CYS | LYS | ASP | ASP | VAL | ASN | ARG | LEU | CYS | ||||
| 3 | ARG | VAL | CYS | ALA | CYS | HIS | LEU | CYS | GLY | GLY | ||||
| 4 | ARG | GLN | ASP | PRO | ASP | LYS | GLN | LEU | MET | CYS | ||||
| 5 | ASP | GLU | CYS | ASP | MET | ALA | PHE | HIS | ILE | TYR | ||||
| 6 | CYS | LEU | ASP | PRO | PRO | LEU | SER | SER | VAL | PRO | ||||
| 7 | SER | GLU | ASP | GLU | TRP | TYR | CYS | PRO | GLU | CYS | ||||
| 8 | ARG | ASN | ASP | ALA |
Samples:
sample_1: PHD finger, [U-13C; U-15N], 0.3 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.150 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView, Johnson, One Moon Scientific - data analysis
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts