BMRB Entry 11454
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11454
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Title: NMR Chemical Shift Assignments for QB domain of Sp1 PubMed: 22855260
Deposition date: 2011-09-26 Original release date: 2012-12-06
Authors: Hiramatsu, Naoko; Hibino, Emi; Matsuzaki, Katsumi; Kuwahara, Jun; Hoshino, Masaru
Citation: Hiramatsu, Naoko; Hibino, Emi; Matsuzaki, Katsumi; Kuwahara, Jun; Hoshino, Masaru. "Interaction between isolated transcriptional activation domains of Sp1 revealed by heteronuclear magnetic resonance" Protein Sci. 21, 1481-1488 (2012).
Assembly members:
QB domain of Sp1, polymer, 172 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX1
Entity Sequences (FASTA):
QB domain of Sp1: GILTNSQGQTPQRVSGLQGS
DALNIQQNQTSGGSLQAGQQ
KEGEQNQQTQQQQILIQPQL
VQGGQALQALQAAPLSGQTF
TTQAISQETLQNLQLQAVPN
SGPIIIRTPTVGPNGQVSWQ
TLQLQNLQVQNPQAQTITLA
PMQGVSLGQTPGGTLEDYKD
DDDKGSHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 304 |
| 15N chemical shifts | 143 |
| 1H chemical shifts | 143 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | QB domain of Sp1 | 1 |
Entities:
Entity 1, QB domain of Sp1 172 residues - Formula weight is not available
| 1 | GLY | ILE | LEU | THR | ASN | SER | GLN | GLY | GLN | THR | ||||
| 2 | PRO | GLN | ARG | VAL | SER | GLY | LEU | GLN | GLY | SER | ||||
| 3 | ASP | ALA | LEU | ASN | ILE | GLN | GLN | ASN | GLN | THR | ||||
| 4 | SER | GLY | GLY | SER | LEU | GLN | ALA | GLY | GLN | GLN | ||||
| 5 | LYS | GLU | GLY | GLU | GLN | ASN | GLN | GLN | THR | GLN | ||||
| 6 | GLN | GLN | GLN | ILE | LEU | ILE | GLN | PRO | GLN | LEU | ||||
| 7 | VAL | GLN | GLY | GLY | GLN | ALA | LEU | GLN | ALA | LEU | ||||
| 8 | GLN | ALA | ALA | PRO | LEU | SER | GLY | GLN | THR | PHE | ||||
| 9 | THR | THR | GLN | ALA | ILE | SER | GLN | GLU | THR | LEU | ||||
| 10 | GLN | ASN | LEU | GLN | LEU | GLN | ALA | VAL | PRO | ASN | ||||
| 11 | SER | GLY | PRO | ILE | ILE | ILE | ARG | THR | PRO | THR | ||||
| 12 | VAL | GLY | PRO | ASN | GLY | GLN | VAL | SER | TRP | GLN | ||||
| 13 | THR | LEU | GLN | LEU | GLN | ASN | LEU | GLN | VAL | GLN | ||||
| 14 | ASN | PRO | GLN | ALA | GLN | THR | ILE | THR | LEU | ALA | ||||
| 15 | PRO | MET | GLN | GLY | VAL | SER | LEU | GLY | GLN | THR | ||||
| 16 | PRO | GLY | GLY | THR | LEU | GLU | ASP | TYR | LYS | ASP | ||||
| 17 | ASP | ASP | ASP | LYS | GLY | SER | HIS | HIS | HIS | HIS | ||||
| 18 | HIS | HIS |
Samples:
sample_1: QB domain of Sp1, [U-99% 15N], 50 – 550 uM; H2O 90%; D2O 10%; NaCl 140 mM; Na-Phosphate 20 mM
sample_2: QB domain of Sp1, [U-99% 13C; U-99% 15N], 500 uM; H2O 90%; D2O 10%; NaCl 140 mM; Na-Phosphate 20 mM
sample_conditions_1: ionic strength: 150 mM; pH: 7.3; pressure: 1 atm; temperature: 277 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
| 3D hNcocaNH | sample_2 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Zhengrong and Bax - processing
NMRDraw, Delaglio, Zhengrong and Bax - data analysis
PIPP, Garrett - peak picking
NMR spectrometers:
- Bruker DMX 600 MHz
Related Database Links:
| DBJ | BAB13476 BAD92915 BAI46444 |
| EMBL | CBM42955 |
| GB | AAA61154 AAF67726 AAH43224 AAH62539 ADL59934 |
| REF | NP_001238754 NP_001253853 NP_001272689 NP_003100 NP_612482 |
| SP | P08047 |
| AlphaFold | P08047 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts