BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11480

Title: Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT

Deposition date: 2012-03-06 Original release date: 2015-01-20

Authors: Tochio, Naoya; Umehara, Takashi; Kigawa, Takanori; Yokoyama, Shigeyuki

Citation: Tochio, Naoya; Umehara, Takashi; Kigawa, Takanori; Yokoyama, Shigeyuki. "Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT"  .

Assembly members:
protein, polymer, 37 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P060718-06

Entity Sequences (FASTA):
protein: GSSGSSGRSYSCPVCEKSFS EDRLIKSHIKTNHPEVS

Data sets:
Data typeCount
13C chemical shifts139
15N chemical shifts28
1H chemical shifts207

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1protein1
2ZINC ION2

Entities:

Entity 1, protein 37 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYARGSERTYR
2   SERCYSPROVALCYSGLULYSSERPHESER
3   GLUASPARGLEUILELYSSERHISILELYS
4   THRASNHISPROGLUVALSER

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: protein, [U-13C; U-15N], 1.14 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; DTT, [U-2H], 1 mM; sodium azide 0.02%; zinc chloride 50 uM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

Kujira, N. Kobayashi - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAA96009 BAD12567 BAD12568 BAD12569 BAG72872
GB AAH12989 AAH25423 AAH53930 AAH98405 AAI01767
REF NP_001025110 NP_001128429 NP_001139360 NP_001161055 NP_001167628
SP Q7TS63 Q9P243
TPG DAA22834
AlphaFold Q7TS63 Q9P243

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts