BMRB Entry 11515
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11515
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Title: goat alpha-lactalbumin at pH 2.0 PubMed: 23580643
Deposition date: 2012-10-30 Original release date: 2017-03-13
Authors: Nakamura, Takashi; Aizawa, Tomoyasu; Demura, Makoto; Kawano, Keiichi; Makabe, Koki; Kuwajima, Kunihiro
Citation: Nakamura, Takashi; Aizawa, Tomoyasu; Kariya, Ryusho; Okada, Seiji; Demura, Makoto; Kawano, Keiichi; Makabe, Koki; Kuwajima, Kunihiro. "Molecular mechanisms of the cytotoxicity of human alpha-lactalbumin made lethal to tumor cells (HAMLET) and other protein-oleic acid complexes" J. Biol. Chem. 288, 14408-14416 (2013).
Assembly members:
goat_alpha-lactalbumin, polymer, 123 residues, Formula weight is not available
Natural source: Common Name: Capra Taxonomy ID: 9922 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: not available Capra
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pSCREEN-LA
Entity Sequences (FASTA):
goat_alpha-lactalbumin: EQLTKCEVFQKLKDLKDYGG
VSLPEWVCTAFHTSGYDTQA
IVQNNDSTEYGLFQINNKIW
CKDDQNPHSRNICNISCDKF
LDDDLTDDIVCAKKILDKVG
INYWLAHKALCSEKLDQWLC
EKL
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 40 |
| 1H chemical shifts | 40 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | goat alpha-lactalbumin | 1 |
Entities:
Entity 1, goat alpha-lactalbumin 123 residues - Formula weight is not available
| 1 | GLU | GLN | LEU | THR | LYS | CYS | GLU | VAL | PHE | GLN | ||||
| 2 | LYS | LEU | LYS | ASP | LEU | LYS | ASP | TYR | GLY | GLY | ||||
| 3 | VAL | SER | LEU | PRO | GLU | TRP | VAL | CYS | THR | ALA | ||||
| 4 | PHE | HIS | THR | SER | GLY | TYR | ASP | THR | GLN | ALA | ||||
| 5 | ILE | VAL | GLN | ASN | ASN | ASP | SER | THR | GLU | TYR | ||||
| 6 | GLY | LEU | PHE | GLN | ILE | ASN | ASN | LYS | ILE | TRP | ||||
| 7 | CYS | LYS | ASP | ASP | GLN | ASN | PRO | HIS | SER | ARG | ||||
| 8 | ASN | ILE | CYS | ASN | ILE | SER | CYS | ASP | LYS | PHE | ||||
| 9 | LEU | ASP | ASP | ASP | LEU | THR | ASP | ASP | ILE | VAL | ||||
| 10 | CYS | ALA | LYS | LYS | ILE | LEU | ASP | LYS | VAL | GLY | ||||
| 11 | ILE | ASN | TYR | TRP | LEU | ALA | HIS | LYS | ALA | LEU | ||||
| 12 | CYS | SER | GLU | LYS | LEU | ASP | GLN | TRP | LEU | CYS | ||||
| 13 | GLU | LYS | LEU |
Samples:
sample_1: goat alpha-lactalbumin, [U-95% 13C; U-95% 15N], 300 uM; D2O 10%; H2O 90%
sample_conditions_1: pH: 2.0; pressure: 1 atm; temperature: 323.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Zhengrong and Bax - processing
NMR spectrometers:
- JEOL ECA 920 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts