BMRB Entry 11524
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11524
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Title: NMR Structure of designed protein, AF.2A1, (Ensembles) PubMed: 24356963
Deposition date: 2013-04-18 Original release date: 2014-01-02
Authors: Watanabe, Hideki; Yamasaki, Kazuhiko; Honda, Shinya
Citation: Watanabe, Hideki; Yamasaki, Kazuhiko; Honda, Shinya. "Structurally guided stepwise segment elongation for the design of a small sized protein: implications for primordial protein evolution" J. Biol. Chem. ., .-. (2013).
Assembly members:
entity, polymer, 25 residues, 2716.968 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity: GVVRQWSGYDPRTGTWRSSI
AYGGG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 155 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 25 residues - 2716.968 Da.
| 1 | GLY | VAL | VAL | ARG | GLN | TRP | SER | GLY | TYR | ASP | ||||
| 2 | PRO | ARG | THR | GLY | THR | TRP | ARG | SER | SER | ILE | ||||
| 3 | ALA | TYR | GLY | GLY | GLY |
Samples:
sample_1: entity 1.8 mM; sodium acetate-d4, [U-100% 2H], 10 mM; D2O 5%; H2O 95%
sample_conditions_1: ionic strength: 10 mM; pH: 4.5; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker AMX 500 MHz
Related Database Links:
| PDB |