BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 11530

Title: chitin binding domain1   PubMed: 24272751

Deposition date: 2013-08-19 Original release date: 2014-04-22

Authors: Uegaki, Koichi

Citation: Mine, Shouhei; Nakamura, Tsutomu; Sato, Takaaki; Ikegami, Takahisa; Uegaki, Koichi. "Solution structure of the chitin-binding domain 1 (ChBD1) of a hyperthermophilic chitinase from Pyrococcus furiosus"  J. Biochem. 155, 115-122 (2014).

Assembly members:
entity, polymer, 73 residues, 8352.153 Da.

Natural source:   Common Name: Pyrococcus furiosus   Taxonomy ID: 2261   Superkingdom: Archaea   Kingdom: not available   Genus/species: Pyrococcus furiosus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet21

Entity Sequences (FASTA):
entity: GPSTSMENLVSLQNLLVYVN WSSYPKYEPGKEYNQGDIVE YNGKLYKAKYWTTSPPSDDP YGSWEYLGEAEPT

Data sets:
Data typeCount
13C chemical shifts302
15N chemical shifts75
1H chemical shifts449

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity1

Entities:

Entity 1, entity 73 residues - 8352.153 Da.

1   GLYPROSERTHRSERMETGLUASNLEUVAL
2   SERLEUGLNASNLEULEUVALTYRVALASN
3   TRPSERSERTYRPROLYSTYRGLUPROGLY
4   LYSGLUTYRASNGLNGLYASPILEVALGLU
5   TYRASNGLYLYSLEUTYRLYSALALYSTYR
6   TRPTHRTHRSERPROPROSERASPASPPRO
7   TYRGLYSERTRPGLUTYRLEUGLYGLUALA
8   GLUPROTHR

Samples:

sample_1: MES, [U-100% 15N], 1 mM; MES, [U-100% 13C; U-100% 15N], 1 mM; MES 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
GB AAL81358 AFN04021
REF WP_011012377

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts