BMRB Entry 11537
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11537
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Title: Solution structure of actinomycin
Deposition date: 2013-10-16 Original release date: 2014-10-20
Authors: Umetsu, Yoshitaka; Ohki, Shinya
Citation: Umetsu, Yoshitaka; Ohki, Shinya. "Solution structure of actinomycin" .
Assembly members:
entity, polymer, 36 residues, 4078.634 Da.
Natural source: Common Name: Actinomyces sp. Taxonomy ID: 29317 Superkingdom: Bacteria Kingdom: not available Genus/species: Actinomyces sp.
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity: GFGCPWNAYECDRHCVSKGY
TGGNCRGKIRQTCHCY
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 189 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 36 residues - 4078.634 Da.
| 1 | GLY | PHE | GLY | CYS | PRO | TRP | ASN | ALA | TYR | GLU | ||||
| 2 | CYS | ASP | ARG | HIS | CYS | VAL | SER | LYS | GLY | TYR | ||||
| 3 | THR | GLY | GLY | ASN | CYS | ARG | GLY | LYS | ILE | ARG | ||||
| 4 | GLN | THR | CYS | HIS | CYS | TYR |
Samples:
sample_1: entity 2 mM; D2O 10%; H2O 90%
sample_conditions_1: pH: 3.0; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance 800 MHz