BMRB Entry 11542

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11542

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for yeast Tim50 PBD domain
Deposition date:
2013-11-12
Original release date:
2014-03-25
Authors:
Rahman, Bytul; Anzai, Takahiro; Kawano, Shin; Endo, Toshiya
Citation:

Citation: Rahman, Bytul; Kawano, Shin; Esaki, Kaori; Anzai, Takahiro; Endo, Toshiya. "NMR analyses on the interactions of the yeast Tim50 C-terminal region with the presequence and Tim50 core domain."  FEBS Lett. 588, 678-684 (2014).
PubMed: 24462684

Assembly members:

Assembly members:
Tim50_PBD, polymer, 72 residues, 8400 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Tim50_PBD: MGSSHHHHHHSSGLVPRGSH MEEGQKNYLMFMKMIEEEKE KIRIQQEQMGGQTFTLKDYV EGNLPSPEEQMK

Data sets:
Data typeCount
13C chemical shifts160
15N chemical shifts55
1H chemical shifts55

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Tim50 PBD1

Entities:

Entity 1, Tim50 PBD 72 residues - 8400 Da.

Residues 379-399 represent a non-native affinity His-tag.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METGLUGLUGLYGLNLYSASNTYRLEUMET
4   PHEMETLYSMETILEGLUGLUGLULYSGLU
5   LYSILEARGILEGLNGLNGLUGLNMETGLY
6   GLYGLNTHRPHETHRLEULYSASPTYRVAL
7   GLUGLYASNLEUPROSERPROGLUGLUGLN
8   METLYS

Related Database Links:

DBJ GAA26910
EMBL CAY86898
GB AAB68302 AHY78114 AJP42082 AJV91172 AJV91610
REF NP_015262
SP Q02776
TPG DAA11368
AlphaFold Q02776

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks