BMRB Entry 11542
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11542
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for yeast Tim50 PBD domain PubMed: 24462684
Deposition date: 2013-11-12 Original release date: 2014-03-25
Authors: Rahman, Bytul; Anzai, Takahiro; Kawano, Shin; Endo, Toshiya
Citation: Rahman, Bytul; Kawano, Shin; Esaki, Kaori; Anzai, Takahiro; Endo, Toshiya. "NMR analyses on the interactions of the yeast Tim50 C-terminal region with the presequence and Tim50 core domain." FEBS Lett. 588, 678-684 (2014).
Assembly members:
Tim50_PBD, polymer, 72 residues, 8400 Da.
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b
Entity Sequences (FASTA):
Tim50_PBD: MGSSHHHHHHSSGLVPRGSH
MEEGQKNYLMFMKMIEEEKE
KIRIQQEQMGGQTFTLKDYV
EGNLPSPEEQMK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 160 |
| 15N chemical shifts | 55 |
| 1H chemical shifts | 55 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Tim50 PBD | 1 |
Entities:
Entity 1, Tim50 PBD 72 residues - 8400 Da.
Residues 379-399 represent a non-native affinity His-tag.
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | MET | GLU | GLU | GLY | GLN | LYS | ASN | TYR | LEU | MET | ||||
| 4 | PHE | MET | LYS | MET | ILE | GLU | GLU | GLU | LYS | GLU | ||||
| 5 | LYS | ILE | ARG | ILE | GLN | GLN | GLU | GLN | MET | GLY | ||||
| 6 | GLY | GLN | THR | PHE | THR | LEU | LYS | ASP | TYR | VAL | ||||
| 7 | GLU | GLY | ASN | LEU | PRO | SER | PRO | GLU | GLU | GLN | ||||
| 8 | MET | LYS |
Samples:
sample_1: Tim50 sPBD, [U-100% 13C; U-100% 15N], 100 uM; potassium phosphate 20 mM; potassium chloride 50 mM; H2O 93%; D2O 7%
sample_conditions_1: ionic strength: 70 mM; pH: 6.7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCANNH | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY v3.114, Goddard - chemical shift assignment
TOPSPIN v1.3, Bruker Biospin - processing
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| DBJ | GAA26910 |
| EMBL | CAY86898 |
| GB | AAB68302 AHY78114 AJP42082 AJV91172 AJV91610 |
| REF | NP_015262 |
| SP | Q02776 |
| TPG | DAA11368 |
| AlphaFold | Q02776 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts