BMRB Entry 11563
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11563
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Refined solution structure of the first RNA recognition motif domain in CPEB3 PubMed: 5066254
Deposition date: 2014-04-15 Original release date: 2016-07-13
Authors: Tsuda, Kengo; Kuwasako, Kanako; Nagata, Takashi; Takahashi, Mari; Kigawa, Takanori; Kobayashi, Naohiro; Guntert, Peter; Shirouzu, Mikako; Yokoyama, Shigeyuki; Muto, Yutaka
Citation: Tsuda, Kengo; Kuwasako, Kanako; Nagata, Takashi; Takahashi, Mari; Kigawa, Takanori; Kobayashi, Naohiro; Guntert, Peter; Shirouzu, Mikako; Yokoyama, Shigeyuki; Muto, Yutaka. "Novel RNA recognition motif domain in the cytoplasmic polyadenylation element binding protein 3" Proteins 82, 2879-2886 (2014).
Assembly members:
The_first_RNA_recognition_motif_domain, polymer, 114 residues, 12428.944 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: pCR2.1
Entity Sequences (FASTA):
The_first_RNA_recognition_motif_domain: GSSGSSGSRKVFVGGLPPDI
DEDEITASFRRFGPLVVDWP
HKAESKSYFPPKGYAFLLFQ
EESSVQALIDACLEEDGKLY
LCVSSPTIKDKPVQIRPWNL
SDSDFVMDSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 493 |
| 15N chemical shifts | 103 |
| 1H chemical shifts | 745 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | The_first_RNA_recognition_motif_domain | 1 |
Entities:
Entity 1, The_first_RNA_recognition_motif_domain 114 residues - 12428.944 Da.
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | SER | ARG | LYS | ||||
| 2 | VAL | PHE | VAL | GLY | GLY | LEU | PRO | PRO | ASP | ILE | ||||
| 3 | ASP | GLU | ASP | GLU | ILE | THR | ALA | SER | PHE | ARG | ||||
| 4 | ARG | PHE | GLY | PRO | LEU | VAL | VAL | ASP | TRP | PRO | ||||
| 5 | HIS | LYS | ALA | GLU | SER | LYS | SER | TYR | PHE | PRO | ||||
| 6 | PRO | LYS | GLY | TYR | ALA | PHE | LEU | LEU | PHE | GLN | ||||
| 7 | GLU | GLU | SER | SER | VAL | GLN | ALA | LEU | ILE | ASP | ||||
| 8 | ALA | CYS | LEU | GLU | GLU | ASP | GLY | LYS | LEU | TYR | ||||
| 9 | LEU | CYS | VAL | SER | SER | PRO | THR | ILE | LYS | ASP | ||||
| 10 | LYS | PRO | VAL | GLN | ILE | ARG | PRO | TRP | ASN | LEU | ||||
| 11 | SER | ASP | SER | ASP | PHE | VAL | MET | ASP | SER | GLY | ||||
| 12 | PRO | SER | SER | GLY |
Samples:
sample_1: The_first_RNA_recognition_motif_domain, [U-100% 13C; U-100% 15N], 1.06 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
xwinnmr v3.5, Bruker Biospin - collection
NMRPipe v20030801, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView v5.0.4, Johnson, One Moon Scientific - data analysis
Kujira v0.9843, Kobayashi, N - data analysis
CYANA v2.0.17, Guntert, Mumenthaler and Wuthrich - structure solution
AMBER v9, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement
NMR spectrometers:
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts