BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 11593

Title: Effects of linker length and transient secondary structure elements in the intrinsically disordered RAM region of NICD on Notch signaling   PubMed: 26344835

Deposition date: 2015-05-21 Original release date: 2016-07-01

Authors: Sherry, Kathryn; Johnson, Scott; Hatem, Christine; Majumdar, Ananya; Barrick, Doug

Citation: Sherry, Kathryn; Johnson, Scott; Hatem, Christine; Majumdar, Ananya; Barrick, Doug. "Effects of Linker Length and Transient Secondary Structure Elements in the Intrinsically Disordered Notch RAM Region on Notch Signaling"  J. Mol. Biol. 427, 3587-3597 (2015).

Assembly members:
RAM, polymer, 133 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24b

Entity Sequences (FASTA):
RAM: MMARKRRRQHGQLWFPEGFK VSEASKKKRREPLGEDSVGL KPLKNASDGALMDDNQNEWG DEDLETKKFRFEEPVVLPDL DDQTDHRQWTQQHLDAADLR MSAMAPTPPQGEVDADCMDV NVRGPDGFTPLLE

Data sets:
Data typeCount
13C chemical shifts282
15N chemical shifts97
1H chemical shifts97

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RAM1

Entities:

Entity 1, RAM 133 residues - Formula weight is not available

1   METMETALAARGLYSARGARGARGGLNHIS
2   GLYGLNLEUTRPPHEPROGLUGLYPHELYS
3   VALSERGLUALASERLYSLYSLYSARGARG
4   GLUPROLEUGLYGLUASPSERVALGLYLEU
5   LYSPROLEULYSASNALASERASPGLYALA
6   LEUMETASPASPASNGLNASNGLUTRPGLY
7   ASPGLUASPLEUGLUTHRLYSLYSPHEARG
8   PHEGLUGLUPROVALVALLEUPROASPLEU
9   ASPASPGLNTHRASPHISARGGLNTRPTHR
10   GLNGLNHISLEUASPALAALAASPLEUARG
11   METSERALAMETALAPROTHRPROPROGLN
12   GLYGLUVALASPALAASPCYSMETASPVAL
13   ASNVALARGGLYPROASPGLYPHETHRPRO
14   LEULEUGLU

Samples:

sample_1: RAM, [U-100% 13C; U-100% 15N], 2 mM; sodium phosphate 25 mM; sodium chloride 50 mM; EDTA 1 mM; TCEP 0.1 mM; H2O 95%; D2O, [U-2H], 5%

sample_conditions_1: ionic strength: 75 mM; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CA)Nsample_1isotropicsample_conditions_1
3D HN(COCA)Nsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts