BMRB Entry 12026

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR12026

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Title:
NMR resonance assignments of the NZF domain of mouse HOIL-1L in free form and linear di-ubiquitin-bound form
Deposition date:
2018-10-19
Original release date:
2018-11-09
Authors:
Ishii, Naoki; Walinda, Erik; Iwakawa, Naoto; Morimoto, Daichi; Iwai, Kazuhiro; Sugase, Kenji; Shirakawa, Masahiro
Citation:

Citation: Ishii, Naoki; Walinda, Erik; Iwakawa, Naoto; Morimoto, Daichi; Iwai, Kazuhiro; Sugase, Kenji; Shirakawa, Masahiro. "NMR resonance assignments of the NZF domain of mouse HOIL-1L in free form and linear di-ubiquitin-bound form"  Biomol. NMR Assignments ., .-..

Assembly members:

Assembly members:
NZF_domain, polymer, 66 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.
linear di-ubiquitin, polymer, 156 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NZF_domain: GPLGSPEPVGWQCPGCTFIN KPTRPGCEMCCRARPETYQI PASYQPDEEERARLAGEEEA LRQYQQ
linear di-ubiquitin: GPLGMQIFVKTLTGKTITLE VEPSDTIENVKAKIQDKEGI PPDQQRLIFAGKQLEDGRTL SDYNIQKESTLHLVLRLRGG MQIFVKTLTGKTITLEVEPS DTIENVKAKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYN IQKESTLHLVLRLRAA

Data sets:
Data typeCount
13C chemical shifts166
15N chemical shifts55
1H chemical shifts55

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HOIL-1L1
2ZN2
3linear di-ubiquitin3

Entities:

Entity 1, HOIL-1L 66 residues - Formula weight is not available

1   GLYPROLEUGLYSERPROGLUPROVALGLY
2   TRPGLNCYSPROGLYCYSTHRPHEILEASN
3   LYSPROTHRARGPROGLYCYSGLUMETCYS
4   CYSARGALAARGPROGLUTHRTYRGLNILE
5   PROALASERTYRGLNPROASPGLUGLUGLU
6   ARGALAARGLEUALAGLYGLUGLUGLUALA
7   LEUARGGLNTYRGLNGLN

Entity 2, ZN - Zn - 65.409 Da.

1   ZN

Entity 3, linear di-ubiquitin 156 residues - Formula weight is not available

1   GLYPROLEUGLYMETGLNILEPHEVALLYS
2   THRLEUTHRGLYLYSTHRILETHRLEUGLU
3   VALGLUPROSERASPTHRILEGLUASNVAL
4   LYSALALYSILEGLNASPLYSGLUGLYILE
5   PROPROASPGLNGLNARGLEUILEPHEALA
6   GLYLYSGLNLEUGLUASPGLYARGTHRLEU
7   SERASPTYRASNILEGLNLYSGLUSERTHR
8   LEUHISLEUVALLEUARGLEUARGGLYGLY
9   METGLNILEPHEVALLYSTHRLEUTHRGLY
10   LYSTHRILETHRLEUGLUVALGLUPROSER
11   ASPTHRILEGLUASNVALLYSALALYSILE
12   GLNASPLYSGLUGLYILEPROPROASPGLN
13   GLNARGLEUILEPHEALAGLYLYSGLNLEU
14   GLUASPGLYARGTHRLEUSERASPTYRASN
15   ILEGLNLYSGLUSERTHRLEUHISLEUVAL
16   LEUARGLEUARGALAALA

Samples:

sample_1: NZF domain, [U-100% 13C; U-100% 15N], 0.5 mM; linear di-ubiquitin 2.0 mM; DTT 1 mM; HEPES 20 mM; sodium chloride 50 mM; H2O 95%; D2O, [U-100% 2H], 5%

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D (H)CC(CO)NHsample_1isotropicsample_conditions_1
3D 15N NOESY-HSQCsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks