BMRB Entry 12036

Title:
Backbone assignment for the segmental-labeled basic region and HMG-box in the DNA-binding domain of Drosophila melanogaster SSRP1
Deposition date:
2019-10-25
Original release date:
2020-07-09
Authors:
Aoki, Daisuke; Uewaki, Jun-ichi; Tochio, Naoya; Tate, Shin-ichi
Citation:

Citation: Aoki, Daisuke; Awazu, Akinori; Fujii, Masashi; Uewaki, Jun-ichi; Hashimoto, Manami; Tochio, Naoya; Umehara, Takashi; Tate, Shin-ichi. "Ultrasensitive Change in Nucleosome Binding by Multiple Phosphorylations to the Intrinsically Disordered Region of the Histone Chaperone FACT."  J. Mol. Biol. ., .-. (2020).
PubMed: 32553729

Assembly members:

Assembly members:
BID-HMG, polymer, 192 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTWIN1-his and pET28a

Data sets:
Data typeCount
13C chemical shifts302
15N chemical shifts94
1H chemical shifts94

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1component 11

Entities:

Entity 1, component 1 192 residues - Formula weight is not available

1   METGLYTYRLYSASPVALASPPHEGLYASP
2   SERASPASNGLUASNGLUPROASPALATYR
3   LEUALAARGLEULYSALAGLUALAARGGLU
4   LYSGLUGLUASPASPASPASPGLYASPSER
5   ASPGLUGLUSERTHRASPGLUASPPHELYS
6   PROASNGLUASNGLUSERASPVALALAGLU
7   GLUTYRASPSERASNVALGLUSERASPSER
8   ASPASPASPSERASPALASERGLYGLYGLY
9   GLYASPSERASPGLYALACYSLYSLYSLYS
10   GLULYSLYSSERGLULYSLYSGLULYSLYS
11   GLULYSLYSHISLYSGLULYSGLUARGTHR
12   LYSLYSPROSERLYSLYSLYSLYSASPSER
13   GLYLYSPROLYSARGALATHRTHRALAPHE
14   METLEUTRPLEUASNASPTHRARGGLUSER
15   ILELYSARGGLUASNPROGLYILELYSVAL
16   THRGLUILEALALYSLYSGLYGLYGLUMET
17   TRPLYSGLULEULYSASPLYSSERLYSTRP
18   GLUASPALAALAALALYSASPLYSGLNARG
19   TYRHISASPGLUMETARGASNTYRLYSPRO
20   GLUALA

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks