Biological Magnetic Resonance Data Bank

A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15056

Title: Antiamoebin-I in methanol solution - rapid exchange between right- and left-handed 3-10-helical conformations   PubMed: 17589862

Deposition date: 2006-11-28 Original release date: 2007-10-16

Authors: Shenkarev, Zakhar; Paramonov, Alexander; Nadezhdin, Kirill

Citation: Shenkarev, Zakhar; Paramonov, Alexander; Nadezhdin, Kirill; Bocharov, E.; Kudelina, I.; Skladnev, D.; Tagaev, A.; Yakimenko, Z.; Ovchinnikova, T.; Arseniev, A.. "Antiamoebin I in methanol solution: rapid exchange between right-handed and left-handed 3(10)-helical conformations"  Chem. Biodivers. 4, 1219-1242 (2007).

Assembly members:
Aam-I, polymer, 17 residues, Formula weight is not available

Natural source:   Common Name: Emericellopsis minima   Taxonomy ID: 45245   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Emericellopsis minima

Experimental source:   Production method: purified from the natural source   Host organism: Emerisellopsis minima

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts69
15N chemical shifts14
1H chemical shifts80

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all


Entity Assembly IDEntity NameEntity ID


Entity 1, Aam-I 17 residues - Formula weight is not available

full sequence - FUUUJGLUUOQJOUPFoh U=AIB (a-aminoisobutyric acid), J=DIV (D-isovaline), O=HYP acetylted N-terminus and a C-terminal a-amino alcohol



sample_1: Aam-I, [U-98% 13C; U-98% 15N], 4 mM

sample_2: Aam-I, [U-98% 15N], 4 mM

sample_3: Aam-I 4 mM

sample_conditions_1: ionic strength: 0 M; pH: 6.2; pressure: 1 atm; temperature: 303 K


NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D HNCAsample_1isotropicsample_conditions_1
2D HNCOsample_1isotropicsample_conditions_1
2D HN(CA)COsample_1isotropicsample_conditions_1
2D HN(CO)CAsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D HNCACBsample_1isotropicsample_conditions_1
2D HCCH-TOCSYsample_1isotropicsample_conditions_1


CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

FANTOM, Braun - molecular modeling, Monte-Carlo simulations

Molmol, Koradi, Billeter and Wuthrich - figure drawnings, visual analysis

XEASY, Bartels et al. - chemical shift assignment, peak picking

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker DRX 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts