BMRB Entry 15102

Name: IKs producing slow voltage-gated potassium channel subunit beta, KCNE1
Published: 2007-10-17

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR15102

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

IKs producing slow voltage-gated potassium channel subunit beta, KCNE1

Deposition date:

2007-01-19

Original release date:

2007-10-17

Authors:

Tian, Changlin; Kim, Hak jun; Sanders, Charles

Citation:

Citation: Tian, Changlin; Vanoye, Carlos; Kang, Congbao; Welch, Richard; Kim, Hak; George, Alfred; Sanders, Charles. "Preparation, Functional Characterization, and NMR Studies of Human KCNE1, a Voltage-Gated Potassium Channel Accessory Subunit Associated with Deafness and Long QT Syndrome(,)" Biochemistry 46, 11459-11472 (2007).
PubMed: 17892302

Assembly members:

Assembly members:
KCNE1, polymer, 129 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET16b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
KCNE1: MILSNTTAVTPFLTKLWQET VQQGGNMSGLARRSPRSGDG KLEALYVLMVLGFFGFFTLG IMLSYIRSKKLEHSNDPFNV YIESDAWQEKDKAYVQARVL ESYKSCYVVENHLAIEQPNT HLPETKPSP

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	336
15N chemical shifts	112
1H chemical shifts	499

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	KCNE1	1

Entities:

Entity 1, KCNE1 129 residues - Formula weight is not available

1

MET

ILE

LEU

SER

ASN

THR

ALA

VAL

THR

2

PRO

PHE

LEU

THR

LYS

LEU

TRP

GLN

GLU

THR

3

VAL

GLN

GLY

ASN

MET

SER

GLY

LEU

4

ALA

ARG

SER

PRO

ARG

SER

GLY

ASP

GLY

5

LYS

LEU

GLU

ALA

LEU

TYR

VAL

LEU

MET

VAL

6

LEU

GLY

PHE

GLY

PHE

THR

LEU

GLY

7

ILE

MET

LEU

SER

TYR

ILE

ARG

SER

LYS

8

LEU

GLU

HIS

SER

ASN

ASP

PRO

PHE

ASN

VAL

9

TYR

ILE

GLU

SER

ASP

ALA

TRP

GLN

GLU

LYS

10

ASP

LYS

ALA

TYR

VAL

GLN

ALA

ARG

VAL

LEU

11

GLU

SER

TYR

LYS

SER

CYS

TYR

VAL

GLU

12

ASN

HIS

LEU

ALA

ILE

GLU

GLN

PRO

ASN

THR

13

HIS

LEU

PRO

GLU

THR

LYS

PRO

SER

PRO

Samples:

sample_1: KCNE1, [U-100% 13C; U-100% 15N; 80% 2H], 1 mM

sample_conditions_1: ionic strength: 0.25 M; pH: 6.0; pressure: 1 atm; temperature: 313 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe v6.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMR spectrometers:

Varian INOVA 900 MHz
Bruker AMX 800 MHz
Bruker AMX 600 MHz

PDB	2K21
DBJ	BAG36319 BAG65181 BAG73568
EMBL	CAG46556
GB	AAA36129 AAA58418 AAA63905 AAD25096 AAH36452
REF	NP_000210 NP_001121140 NP_001121141 NP_001121142 NP_001257331
SP	P15382
AlphaFold	P15382