BMRB Entry 15104

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15104
MolProbity Validation Chart

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Title:
Structure of NOWA cysteine rich domain 8
Deposition date:
2007-01-22
Original release date:
2007-10-17
Authors:
Meier, S.; Jensen, P.; Adamczyk, P.; Bachinger, H.; Holstein, T.; Engel, J.; Ozbek, S.; Grzesiek, S.
Citation:

Citation: Meier, S.; Jensen, P.; Adamczyk, P.; Bachinger, H.; Holstein, T.; Engel, J.; Oezbek, S.; Grzesiek, S.. "Sequence-structure and structure-function analysis in cysteine-rich domains forming the ultrastable nematocyst wall"  J. Mol. Biol. 368, 718-728 (2007).
PubMed: 17362991

Assembly members:

Assembly members:
Nematocyst_outer_wall_antigen, polymer, 30 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Hydra vulgaris   Taxonomy ID: 6087   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Hydra vulgaris

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEV2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Nematocyst_outer_wall_antigen: GSAQNPCSLQQPGCSSACAP ACRLSCCSLG

Data sets:
Data typeCount
15N chemical shifts26
1H chemical shifts26

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1NW81

Entities:

Entity 1, NW8 30 residues - Formula weight is not available

1   GLYSERALAGLNASNPROCYSSERLEUGLN
2   GLNPROGLYCYSSERSERALACYSALAPRO
3   ALACYSARGLEUSERCYSCYSSERLEUGLY

Samples:

sample: Nematocyst outer wall antigen NW8, [U-100% 15N], 0.5 mM; D2O, [U-2H], 5%; potassium phosphate 5 mM

sample_conditions_1: ionic strength: 20 mM; pH: 5.5; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsampleisotropicsample_conditions_1
2D TOCSYsampleisotropicsample_conditions_1
3D 15N-separated ROESYsampleisotropicsample_conditions_1
15N HSQCsampleisotropicsample_conditions_1

Software:

CNS v1.0, Brunger A. T. et al - refinement

NMRPipe v2.1, Delaglio et al - processing

xwinnmr v3.5, BRUKER - collection

PIPP v4.3.2, Garrett D. et al - data analysis

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker DRX 600 MHz

Related Database Links:

PDB
GB AAN52336
REF NP_001274304

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks