BMRB Entry 15104
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15104
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NMR-STAR v3 text file.
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Title: Structure of NOWA cysteine rich domain 8 PubMed: 17362991
Deposition date: 2007-01-22 Original release date: 2007-10-17
Authors: Meier, S.; Jensen, P.; Adamczyk, P.; Bachinger, H.; Holstein, T.; Engel, J.; Ozbek, S.; Grzesiek, S.
Citation: Meier, S.; Jensen, P.; Adamczyk, P.; Bachinger, H.; Holstein, T.; Engel, J.; Oezbek, S.; Grzesiek, S.. "Sequence-structure and structure-function analysis in cysteine-rich domains forming the ultrastable nematocyst wall" J. Mol. Biol. 368, 718-728 (2007).
Assembly members:
Nematocyst_outer_wall_antigen, polymer, 30 residues, Formula weight is not available
Natural source: Common Name: Hydra vulgaris Taxonomy ID: 6087 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Hydra vulgaris
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEV2
Entity Sequences (FASTA):
Nematocyst_outer_wall_antigen: GSAQNPCSLQQPGCSSACAP
ACRLSCCSLG
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 26 |
| 1H chemical shifts | 26 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NW8 | 1 |
Entities:
Entity 1, NW8 30 residues - Formula weight is not available
| 1 | GLY | SER | ALA | GLN | ASN | PRO | CYS | SER | LEU | GLN | |
| 2 | GLN | PRO | GLY | CYS | SER | SER | ALA | CYS | ALA | PRO | |
| 3 | ALA | CYS | ARG | LEU | SER | CYS | CYS | SER | LEU | GLY |
Samples:
sample: Nematocyst outer wall antigen NW8, [U-100% 15N], 0.5 mM; D2O, [U-2H], 5%; potassium phosphate 5 mM
sample_conditions_1: ionic strength: 20 mM; pH: 5.5; pressure: 1 atm; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample | isotropic | sample_conditions_1 |
| 2D TOCSY | sample | isotropic | sample_conditions_1 |
| 3D 15N-separated ROESY | sample | isotropic | sample_conditions_1 |
| 15N HSQC | sample | isotropic | sample_conditions_1 |
Software:
CNS v1.0, Brunger A. T. et al - refinement
NMRPipe v2.1, Delaglio et al - processing
xwinnmr v3.5, BRUKER - collection
PIPP v4.3.2, Garrett D. et al - data analysis
NMR spectrometers:
- Bruker DRX 800 MHz
- Bruker DRX 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts