BMRB Entry 15143
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15143
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: 1H (NH, HA, HB) Chemical Shift Assignments of the Non-Specific Lipid Transfer Protein (nsLTP1) with post translational modification, isolated from Barley Seeds PubMed: 17854802
Deposition date: 2007-02-24 Original release date: 2007-10-30
Authors: Wijesinha-Bettoni, Ramani; Gao, Chunli; Jenkins, John; Mackie, Alan; Wilde, Peter; Mills, E. N; Smith, Lorna
Citation: Wijesinha-Bettoni, Ramani; Gao, Chunli; Jenkins, John; Mackie, Alan; Wilde, Peter; Mills, E. N; Smith, Lorna. "Post-translational modification of barley LTP1b: the lipid adduct lies in the hydrophobic cavity and alters the protein dynamics" FEBS Lett. 581, 4557-4561 (2007).
Assembly members:
nsLTP1b, polymer, 91 residues, Formula weight is not available
CHDA, non-polymer, 294.3 Da.
Natural source: Common Name: barley Taxonomy ID: 4513 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Hordeum vulgare
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
nsLTP1b: LNCGQVDSKMKPCLTYVQGG
PGPSGECCNGVRDLHNQAQS
SGDRQTVCNCLKGIARGIHN
LNLNNAASIPSKCNVNVPYT
ISPDIDCSRIY
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 279 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | barley lipid transfer protein with post-transaltional modification | 1 |
| 2 | cis-7-heptadecenoic acid | 2 |
Entities:
Entity 1, barley lipid transfer protein with post-transaltional modification 91 residues - Formula weight is not available
| 1 | LEU | ASN | CYS | GLY | GLN | VAL | ASP | SER | LYS | MET | ||||
| 2 | LYS | PRO | CYS | LEU | THR | TYR | VAL | GLN | GLY | GLY | ||||
| 3 | PRO | GLY | PRO | SER | GLY | GLU | CYS | CYS | ASN | GLY | ||||
| 4 | VAL | ARG | ASP | LEU | HIS | ASN | GLN | ALA | GLN | SER | ||||
| 5 | SER | GLY | ASP | ARG | GLN | THR | VAL | CYS | ASN | CYS | ||||
| 6 | LEU | LYS | GLY | ILE | ALA | ARG | GLY | ILE | HIS | ASN | ||||
| 7 | LEU | ASN | LEU | ASN | ASN | ALA | ALA | SER | ILE | PRO | ||||
| 8 | SER | LYS | CYS | ASN | VAL | ASN | VAL | PRO | TYR | THR | ||||
| 9 | ILE | SER | PRO | ASP | ILE | ASP | CYS | SER | ARG | ILE | ||||
| 10 | TYR |
Entity 2, cis-7-heptadecenoic acid - C17 H28 05 - 294.3 Da.
| 1 | CHDA |
Samples:
sample_1: nsLTP1b 4 ± 0.2 mM
sample_conditions_1: pH: 4.0; temperature: 310 K
sample_conditions_2: pH: 3; temperature: 299 K
sample_conditions_3: pH: 5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_2 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_2 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_3 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_3 |
Software:
SPARKY vVersion 3.110, Goddard - data analysis
NMR spectrometers:
- Home-built . 600 MHz