BMRB Entry 15161
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15161
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: The MYND domain from human AML1-ETO PubMed: 17560331
Deposition date: 2007-03-06 Original release date: 2007-10-24
Authors: Liu, Yizhou; Chen, Wei; Gaudet, Justin; Cheney, Matthew; Roudaia, Liya; Cierpicki, Tomasz; Klet, Rachel; Hartman, Kari; Laue, Thomas; Speck, Nancy; Bushweller, John
Citation: Liu, Yizhou; Chen, Wei; Gaudet, Justin; Cheney, Matthew; Roudaia, Liya; Cierpicki, Tomasz; Klet, Rachel; Hartman, Kari; Laue, Thomas; Speck, Nancy; Bushweller, John. "Structural basis for recognition of SMRT/N-CoR by the MYND domain and its contribution to AML1/ETO's activity" Cancer Cell 11, 483-497 (2007).
Assembly members:
ETO_MYND, polymer, 50 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX4T3
Entity Sequences (FASTA):
ETO_MYND: DSSESCWNCGRKASETCSGC
NTARYCGSFCQHKDWEKHHH
ICGQTLQAQQ
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 202 |
| 15N chemical shifts | 59 |
| 1H chemical shifts | 269 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ETO MYND | 1 |
| 2 | ZINC ION, 1 | 2 |
| 3 | ZINC ION, 2 | 2 |
Entities:
Entity 1, ETO MYND 50 residues - Formula weight is not available
| 1 | ASP | SER | SER | GLU | SER | CYS | TRP | ASN | CYS | GLY | |
| 2 | ARG | LYS | ALA | SER | GLU | THR | CYS | SER | GLY | CYS | |
| 3 | ASN | THR | ALA | ARG | TYR | CYS | GLY | SER | PHE | CYS | |
| 4 | GLN | HIS | LYS | ASP | TRP | GLU | LYS | HIS | HIS | HIS | |
| 5 | ILE | CYS | GLY | GLN | THR | LEU | GLN | ALA | GLN | GLN |
Entity 2, ZINC ION, 1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: ETO MYND, [U-99% 13C; U-99% 15N], 1 mM; ZINC ION 1 mM; Bis-Tris 25 mM; NaCl 100 mM; DTT 5 mM; D2O 5%
sample_conditions_1: ionic strength: 0.1 M; pH: 6; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Varian Unity 600 MHz
Related Database Links:
| BMRB | 15173 |
| PDB | |
| DBJ | BAA03089 BAA03247 BAA03558 BAA06774 BAA07755 |
| EMBL | CAA56311 CAF99400 CAG33073 |
| GB | AAB34819 AAC28931 AAC28932 AAF72198 AAF72199 |
| REF | NP_001070244 NP_001089065 NP_001102127 NP_001104496 NP_001104497 |
| SP | Q06455 Q61909 |
| AlphaFold | Q06455 Q61909 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts