BMRB Entry 15174

Name: 1H, alpha-13C Chemical Shift Assignments for [L-Phe44]iota-RXIA
Published: 2007-10-24

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PDB ID: 2p4l
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR15174
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, alpha-13C Chemical Shift Assignments for [L-Phe44]iota-RXIA

Deposition date:

2007-03-15

Original release date:

2007-10-24

Authors:

Buczek, Olga; Wei, Daxiu; Babon, Jeffrey; Yang, Xiaodong; Fiedler, Brain; Yoshikami, Doju; Olivera, Baldomero; Bulaj, Grzegorz; Norton, Raymond

Citation:

Citation: Buczek, Olga; Wei, Daxiu; Babon, Jeffrey; Yang, Xiaodong; Fiedler, Brain; Chen, P.; Yoshikami, Doju; Olivera, Baldomero; Bulaj, Grzegorz; Norton, Raymond. "Structure and sodium channel activity of an excitatory I1-superfamily conotoxin." Biochemistry 46, 9929-9940 (2007).
PubMed: 17696362

Assembly members:

Assembly members:
[L-Phe44]iota-RXIA_(r11a), polymer, 46 residues, Formula weight is not available

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
[L-Phe44]iota-RXIA_(r11a): GXSFCKADEKXCEYHADCCN CCLSGICAXSTNWILPGCST SSFFKI

Data sets:

assigned_chemical_shifts
- chemical-shift
coupling_constants
- coupling_constant_list

Data type	Count
13C chemical shifts	44
1H chemical shifts	274
coupling constants	33

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	iota-RXIA	1

Entities:

Entity 1, iota-RXIA 46 residues - Formula weight is not available

1

GLY

HYP

SER

PHE

CYS

LYS

ALA

ASP

GLU

LYS

2

HYP

CYS

GLU

TYR

HIS

ALA

ASP

CYS

ASN

3

CYS

LEU

SER

GLY

ILE

CYS

ALA

HYP

SER

4

THR

ASN

TRP

ILE

LEU

PRO

GLY

CYS

SER

THR

5

SER

PHE

LYS

ILE

Samples:

(L-Phe44)iota-RXIA_(r11a)sample: [L-Phe44]iota-RXIA (r11a) 1.1 mM; H2O 95%; D2O 5%

(L-Phe44)iota-RXIA: pH: 5.9; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	(L-Phe44)iota-RXIA_(r11a)sample	isotropic	(L-Phe44)iota-RXIA
2D 1H-1H TOCSY	(L-Phe44)iota-RXIA_(r11a)sample	isotropic	(L-Phe44)iota-RXIA
2D DQF-COSY	(L-Phe44)iota-RXIA_(r11a)sample	isotropic	(L-Phe44)iota-RXIA
2D 1H-13C HSQC	(L-Phe44)iota-RXIA_(r11a)sample	isotropic	(L-Phe44)iota-RXIA

Software:

XEASY, Bartels et al. - chemical shift assignment

NMR spectrometers:

Bruker DRX 600 MHz
Bruker Avance 800 MHz