BMRB Entry 15196
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15196
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Citation: Matsui, T.; Kodera, Y.; Endoh, H.; Miyauchi, E.; Komatsu, H.; Sato, T.; Tanaka, T.; Kohno, T.; Maeda, T.. "RNA recognition mechanism of the minimal active domain of the human immunodeficiency virus type-2 nucleocapsid protein" J. Biochem. 141, 269-277 (2007).
PubMed: 17202191
Assembly members:
Gag-Pol_polyprotein_(Pr160Gag-Pol), polymer, 27 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: HIV-2 Taxonomy ID: 11709 Superkingdom: Viruses Kingdom: not available Genus/species: Lentivirus HIV-2
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Gag-Pol_polyprotein_(Pr160Gag-Pol): RAPRRQGCWKCGKTGHVMAK
CPERQAG
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 163 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | polymer chain | 1 |
| 2 | Zinc ion | 2 |
Entities:
Entity 1, polymer chain 27 residues - Formula weight is not available
| 1 | ARG | ALA | PRO | ARG | ARG | GLN | GLY | CYS | TRP | LYS | ||||
| 2 | CYS | GLY | LYS | THR | GLY | HIS | VAL | MET | ALA | LYS | ||||
| 3 | CYS | PRO | GLU | ARG | GLN | ALA | GLY |
Entity 2, Zinc ion - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample: Gag-Pol polyprotein (Pr160Gag-Pol) 5 mM; ZINC ION 5.5 mM; DSS 0.5 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 5.8; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQF-COSY | sample | isotropic | sample_conditions_1 |
| 2D TOCSY | sample | isotropic | sample_conditions_1 |
| 2D NOESY | sample | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH v2.9.9, C.D.SCHWIETERS. - structure solution
NMR spectrometers:
- Bruker DMX 500 MHz