BMRB Entry 15221
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15221
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Title: Solution structure of Tc32 toxin from the scorpion Tityus cambridgei
Deposition date: 2007-04-20 Original release date: 2012-08-03
Authors: Stehling, Eliana; Sforca, Mauricio; Zanchin, Nilson; Pertinhez, Thelma; Spisni, Alberto
Citation: Stehling, Eliana; Sforca, Mauricio; Zanchin, Nilson; Belluzzi, Ottorino; Pertinhez, Thelma; Spisni, Alberto. "Solution structure of Tc32 toxin from the scorpion Tityus cambridgei" .
Assembly members:
Tc32, polymer, 37 residues, Formula weight is not available
Natural source: Common Name: Tityus cambridgei Taxonomy ID: 184226 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Tityus cambridgei
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21d modified
Entity Sequences (FASTA):
Tc32: GSTGPQTTCQAAMCEAGCKG
LGKSMESCQGDTCKCKA
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 197 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | tc32 | 1 |
Entities:
Entity 1, tc32 37 residues - Formula weight is not available
| 1 | GLY | SER | THR | GLY | PRO | GLN | THR | THR | CYS | GLN | ||||
| 2 | ALA | ALA | MET | CYS | GLU | ALA | GLY | CYS | LYS | GLY | ||||
| 3 | LEU | GLY | LYS | SER | MET | GLU | SER | CYS | GLN | GLY | ||||
| 4 | ASP | THR | CYS | LYS | CYS | LYS | ALA |
Samples:
sample_1: Toxin 0.6 mM
sample_conditions_1: pH: 4; pressure: 1 atm; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - data analysis
DYANA, Guntert, Braun and Wuthrich - structure solution
DISCOVER, Accelrys Software Inc. - minimization energy
NMR spectrometers:
- Varian INOVA 600 MHz