BMRB Entry 15241
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15241
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Title: 1H Chemical shift assignments for [Nle15] gastrin-17 in the presence of DPC micelles. PubMed: 17698249
Deposition date: 2007-05-08 Original release date: 2007-10-29
Authors: Stone, Shane; Mierke, Dale; Jackson, Graham
Citation: Stone, Shane; Mierke, Dale; Jackson, Graham. "Evidence for a C-terminal Structural Motif in Gastrin and its Bioactive Fragments in Membrane Mimetic Media." Peptides 28, 1561-1571 (2007).
Assembly members:
Nle15_Gastrin-17, polymer, 18 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Nle15_Gastrin-17: XGPWLEEEEEAYGWXDFX
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 183 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | [Nle15] gastrin-17, major | 1 |
| 2 | [Nle15] gastrin-17, minor | 1 |
Entities:
Entity 1, [Nle15] gastrin-17, major 18 residues - Formula weight is not available
| 1 | PCA | GLY | PRO | TRP | LEU | GLU | GLU | GLU | GLU | GLU | ||||
| 2 | ALA | TYR | GLY | TRP | NLE | ASP | PHE | NH2 |
Samples:
sample: TSP 0.1 mM; [Nle15] Gastrin-17 1.5 mM; phosphate buffer 50 mM; DPC, [U-99% 2H], 150 mM; H2O 90%; D2O, [U-99% 2H], 10%
sample_conditions_1: ionic strength: 50 mM; pH: 5.2; pressure: 1 atm; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz