BMRB Entry 15305
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15305
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Title: 1H Chemical Shift Assignments for Cardiotoxin A3 from Naja Atra at Neutral pH and temperature of 318K PubMed: 17918958
Deposition date: 2007-06-15 Original release date: 2008-02-28
Authors: Tjong, Siu-Cin; Wu, Po-Long; Wang, Chang-Mao; Huang, Wei-Ning; Ho, Nan-Lu; Wu, Wen-Guey
Citation: Tjong, Siu-Cin; Wu, Po-Long; Wang, Chang-Mao; Huang, Wei-Ning; Ho, Nan-Lu; Wu, Wen-Guey. "Role of glycosphingolipid conformational change in membrane pore forming activity of cobra cardiotoxin" Biochemistry 46, 12111-12123 (2007).
Assembly members:
cardiotoxin_A3, polymer, 60 residues, Formula weight is not available
Natural source: Common Name: Chinese cobra Taxonomy ID: 8656 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Naja atra
Experimental source: Production method: purified from the natural source Host organism: Naja atra
Entity Sequences (FASTA):
cardiotoxin_A3: LKCNKLVPLFYKTCPAGKNL
CYKMFMVATPKVPVKRGCID
VCPKSSLLVKYVCCNTDRCN
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 1102 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cardiotoxin A3 | 1 |
Entities:
Entity 1, cardiotoxin A3 60 residues - Formula weight is not available
| 1 | LEU | LYS | CYS | ASN | LYS | LEU | VAL | PRO | LEU | PHE | |
| 2 | TYR | LYS | THR | CYS | PRO | ALA | GLY | LYS | ASN | LEU | |
| 3 | CYS | TYR | LYS | MET | PHE | MET | VAL | ALA | THR | PRO | |
| 4 | LYS | VAL | PRO | VAL | LYS | ARG | GLY | CYS | ILE | ASP | |
| 5 | VAL | CYS | PRO | LYS | SER | SER | LEU | LEU | VAL | LYS | |
| 6 | TYR | VAL | CYS | CYS | ASN | THR | ASP | ARG | CYS | ASN |
Samples:
CTX_A3: cardiotoxin A3 1 mM; phosphate buffer 10 mM
CTX_A3_in_SGC_DPC_micelle: cardiotoxin A3 1 mM; DPC, [U-2H], 45 mM; sulfatide (SGC) 5 mM; phosphate buffer 10 mM
CTX_A3__in_DPC_micelle: cardiotoxin A3 1 mM; DPC, [U-2H], 50 mM; phosphate buffer 10 mM
sample_conditions_1: ionic strength: 10 mM; pH: 6.0; pressure: 1 atm; temperature: 318 K
sample_conditions_2: ionic strength: 19 mM; pH: 6.0; pressure: 1 atm; temperature: 318 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | CTX_A3 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | CTX_A3 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | CTX_A3_in_SGC_DPC_micelle | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | CTX_A3__in_DPC_micelle | isotropic | sample_conditions_2 |
Software:
xwinnmr, Bruker Biospin - chemical shift assignment
NMR spectrometers:
- Bruker DMX 500 MHz